Maiz-Fernández Sheila, Pérez-Álvarez Leyre, Ruiz-Rubio Leire, Pérez González Raúl, Sáez-Martínez Virginia, Ruiz Pérez Jesica, Vilas-Vilela José Luis
Macromolecular Chemistry Group (LABQUIMAC), Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country, UPV/EHU, Barrio Sarriena, s/n 48940 Leioa, Spain.
BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain.
Polymers (Basel). 2019 Apr 24;11(4):742. doi: 10.3390/polym11040742.
Stable hyaluronic acid nanogels were obtained following the water-in-oil microemulsion method by covalent crosslinking with three biocompatible crosslinking agents: Divinyl sulfone, 1,4-butanediol diglycidyl ether (BDDE), and poly(ethylene glycol) bis(amine). All nanoparticles showed a pH-sensitive swelling behavior, according to the pKa value of hyaluronic acid, as a consequence of the ionization of the carboxylic moieties, as it was corroborated by zeta potential measurements. QELS studies were carried out to study the influence of the chemical structure of the crosslinking agents on the particle size of the obtained nanogels. In addition, the effect of the molecular weight of the biopolymer and the degree of crosslinking on the nanogels dimensions was also evaluated for BDDE crosslinked nanoparticles, which showed the highest pH-responsive response.
二乙烯基砜、1,4-丁二醇二缩水甘油醚（BDDE）和聚乙二醇双胺进行共价交联，采用油包水微乳液法获得了稳定的透明质酸纳米凝胶。根据透明质酸的pKa值，由于羧基部分的电离，所有纳米颗粒均表现出pH敏感的溶胀行为，zeta电位测量证实了这一点。进行了准弹性光散射（QELS）研究，以研究交联剂的化学结构对所得纳米凝胶粒径的影响。此外，还评估了生物聚合物分子量和交联度对BDDE交联纳米颗粒尺寸的影响，该纳米颗粒表现出最高的pH响应。
