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从凝乳中分离和鉴定产胞外多糖菌株。

Isolation and characterization of exopolysaccharide-producing strains of from curd.

作者信息

Ali Khubaib, Mehmood Muhammad Huzaifa, Iqbal Muhammad Ahmad, Masud Tariq, Qazalbash Mudassir, Saleem Shahzad, Ahmed Sheraz, Tariq Muhammad Rizwan, Safdar Waseem, Nasir Muhammad Adnan, Saeed Muhammad Tariq, Muhammad Abid, Sheas Muhammad Naveed

机构信息

Department of Diet & Nutritional Sciences University of Lahore Islamabad Pakistan.

Faisalabad Medical University Faisalabad Pakistan.

出版信息

Food Sci Nutr. 2019 Mar 3;7(4):1207-1213. doi: 10.1002/fsn3.905. eCollection 2019 Apr.

Abstract

Curd is the most widespread traditional fermented milk product used by a large population and is a good source of vitamin B, protein, and calcium. In this study, the isolation of exopolysaccharide (EPS)-producing strains of subsp. from curd samples was carried out. Identification of EPS-producing strains was done by Gram staining, catalase activity, sugar fermentation test, API 50 CHL, and PCR analysis. These EPS-producing strains were subjected for the estimation of technological properties such as titratable acidity, curdling time, acidification rate, and texture. The strains best in their technological properties were selected for the production of yogurt in combination with EPS- or non-EPS-producing strains of The EPS concentration range was from 41 to 268 mg/L in the yogurt. The highest value of EPS concentration was detected in and non-EPS-producing after 14 days of storage.

摘要

凝乳是广大人群使用的最普遍的传统发酵乳制品,是维生素B、蛋白质和钙的良好来源。在本研究中,从凝乳样品中分离出了亚种的产胞外多糖(EPS)菌株。通过革兰氏染色、过氧化氢酶活性、糖发酵试验、API 50 CHL和PCR分析对产EPS菌株进行了鉴定。对这些产EPS菌株进行了可滴定酸度、凝乳时间、酸化速率和质地等工艺特性的评估。选择工艺特性最佳的菌株与亚种的产EPS或不产EPS菌株组合用于酸奶生产。酸奶中EPS浓度范围为41至268mg/L。在储存14天后,在和不产EPS的中检测到EPS浓度的最高值。

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