State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Department of Biological Sciences, Kent State University, Kent, 44242 Ohio, USA.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2896-2900. doi: 10.1099/ijsem.0.004116. Epub 2020 Mar 25.
A novel bacterium, designated strain SYSU M00256-3, was isolated from a water sample collected from Pearl River Estuary at Guangzhou, PR China. Its taxonomic position was determined by using a polyphasic approach. Cells of the strain were Gram-staining-negative, motile, aerobic and rod-shaped with peritrichous flagella. It could grow at 15-45 °C, pH 4.0-10.0 and in the presence of 0-7.5 % (w/v) NaCl. The chemotaxonomic features of strain SYSU M00256-3 included ubiquinone-10 (Q-10) as the sole respiratory quinone; phosphatidylcholine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as major polar lipids; summed feature 8 (C ω and C 6) as the predominant fatty acids (>70 %). On the basis of 16S rRNA gene sequence analysis, strain SYSU M00256-3 was most closely related to the type strains of CGMCC 1.12584 (97.7 %) and CGMCC 1.12426 (97.2 %), KCTC 52373 (96.7 %), CGMCC 1.12583 (96.4 %). The average nucleotide identity (ANI) values between SYSU M00256-3 and CGMCC 1.12584, CGMCC 1.12426, CGMCC 1.12583 and KCTC 52373 were 78.0, 78.2, 77.7 and 78.8, and the dDDH value is 20.0, 20.8, 20.1 and 20.6 correspondingly. Based on the analyses of the phenotypic, genotypic and phylogenetic characteristics, strain SYSU M00256-3 is characterized to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU M00256-3 (=NBRC 112946=CGMCC 1.16156).
一株新的细菌，命名为 SYSU M00256-3 菌株，从中国广州珠江口采集的水样中分离得到。它的分类地位是通过多相研究确定的。该菌株的细胞革兰氏染色阴性，运动，需氧，杆状，周身鞭毛。它可以在 15-45°C、pH4.0-10.0 和 0-7.5%（w/v）NaCl 的条件下生长。SYSU M00256-3 菌株的化感分类特征包括泛醌-10（Q-10）作为唯一的呼吸醌；磷脂酰胆碱、磷脂酰甲乙醇胺、磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的磷脂作为主要极性脂；特征脂肪酸总和 8（Cω和 C6）为主要脂肪酸（>70%）。基于 16S rRNA 基因序列分析，SYSU M00256-3 与模式菌株 CGMCC 1.12584（97.7%）和 CGMCC 1.12426（97.2%）、KCTC 52373（96.7%）、CGMCC 1.12583（96.4%）最为密切相关。SYSU M00256-3 与 CGMCC 1.12584、CGMCC 1.12426、CGMCC 1.12583 和 KCTC 52373 的平均核苷酸同一性（ANI）值分别为 78.0、78.2、77.7 和 78.8，而 dDDH 值分别为 20.0、20.8、20.1 和 20.6。基于表型、基因型和系统发育特征的分析，SYSU M00256-3 代表了属的一个新种，因此建议采用新种的名称。该模式菌株为 SYSU M00256-3（=NBRC 112946=CGMCC 1.16156）。
