Institute of Nutritional Medicine, University Medical Center Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.
Phytochemistry and Healthy Food Lab, Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Sciences and Technology, CEBAS-CSIC, University Campus of Espinardo, Edif. 25, 30100 Murcia, Spain.
Molecules. 2019 Apr 25;24(8):1632. doi: 10.3390/molecules24081632.
A new method using ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) methodology was developed for the determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) contents in liquid and solid vegetable samples. The advantages of this method are speed, high sensitivity and practical application. In accordance with these advantages, the present method allows the simultaneous determination of AA and DHAA without previous reduction/derivatization of DHAA and without the use of internal standards in the samples. This is of high interest in routine analysis, providing a simpler sample preparation, as well as enhanced accuracy and robustness. Its validation included selectivity, sensitivity and linearity, precision and accuracy, matrix effect, and recovery. The results showed high selectivity and sensitivity, with calibration curves ranging from 10 to 500 ng mL and from 50 to 500 ng mL for AA and DHAA, respectively. Appropriate dilutions for each sample are necessary to avoid the matrix effect with accepted recoveries.
建立了一种新的超高效液相色谱-三重四极杆串联质谱法（UHPLC-QqQ-MS/MS），用于测定液体和固体蔬菜样品中抗坏血酸（AA）和脱氢抗坏血酸（DHAA）的含量。该方法的优点是速度快、灵敏度高、实用性强。根据这些优点，本方法可以在不进行 DHAA 还原/衍生化且不使用内标物的情况下，同时测定 AA 和 DHAA。这在常规分析中非常有意义，因为它提供了更简单的样品制备方法，以及更高的准确性和稳健性。其验证包括选择性、灵敏度和线性、精密度和准确度、基质效应和回收率。结果表明，该方法具有较高的选择性和灵敏度，AA 和 DHAA 的校准曲线范围分别为 10-500ng/mL 和 50-500ng/mL。对于每种样品，都需要进行适当的稀释，以避免接受的回收率的基质效应。
