Fu Xiaochen, Xue Chao, Wang Xin, Wang Aiyu, Zhu Yanwei, Yang Yuanxue, Zhang Yun, Zhou Yun, Zhao Ming, Shan Chenggang, Zhang Jianhua
School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, China.
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, China.
Front Physiol. 2024 Dec 6;15:1478869. doi: 10.3389/fphys.2024.1478869. eCollection 2024.
is an important economic pest affecting Caprifoliaceae and Apiaceae plants, and chemical control is still the main effective control method in the field. Afidopyropen is a new type of pyridine cyclopropyl insecticide, which can effectively control piercing-sucking mouthparts pests and is suitable for pest resistance management. However, the detoxification mechanism of . to afidopyropen is still poorly cleared.
The insecticidal activity of afidopyropen against and the enzyme activity assay and synergism bioassay were evaluated. The detoxification enzyme genes were obtained by transcriptome and validated by quantitative real-time PCR (RT-qPCR). Furthermore, RNA interference was used to study the functions of detoxification enzyme genes.
The activities of cytochrome P450 monooxygenases (P450s) and carboxylesterases (CarEs) were significantly increased under afidopyropen treatment. The toxicity of afidopyropen against was significantly increased after application the inhibitors of piperonyl butoxide and triphenyl phosphate. Sixteen P450 genes and three CarE genes were identified in the transcriptome of . The RT-qPCR results showed that eleven P450 genes and two CarE genes were significantly upregulated under afidopyropen treatment, and the expression of and was upregulated by more than 2.5 times. The expression pattern of and was further analyzed in different developmental stages of and knockdown of and significantly increased the susceptibility of to afidopyropen.
The results of this study uncover the key functions of and in the detoxification mechanism of to afidopyropen, and provide a theoretical basis for the scientific use of afidopyropen in the field.
是一种影响忍冬科和伞形科植物的重要经济害虫，化学防治仍是田间主要的有效防治方法。阿维菌素是一种新型吡啶环丙基杀虫剂，能有效防治刺吸式口器害虫，适用于害虫抗性治理。然而，对阿维菌素的解毒机制仍不清楚。
评估了阿维菌素对的杀虫活性，并进行了酶活性测定和增效生物测定。通过转录组获得解毒酶基因，并通过实时定量PCR（RT-qPCR）进行验证。此外，利用RNA干扰研究解毒酶基因的功能。
在阿维菌素处理下，细胞色素P450单加氧酶（P450s）和羧酸酯酶（CarEs）的活性显著增加。施用胡椒基丁醚和磷酸三苯酯抑制剂后，阿维菌素对的毒性显著增加。在的转录组中鉴定出16个P450基因和3个CarE基因。RT-qPCR结果表明，在阿维菌素处理下，11个P450基因和2个CarE基因显著上调，和的表达上调超过2.5倍。在的不同发育阶段进一步分析了和的表达模式，敲低和显著增加了对阿维菌素的敏感性。
本研究结果揭示了和在对阿维菌素解毒机制中的关键作用，为阿维菌素在田间的科学使用提供了理论依据。
