Tonin Alexandre Alberto, da Silva Aleksandro Schafer, Thorstenberg Maria Luiza, Castilhos Lívia Gelain, França Raqueli Teresinha, Leal Daniela Bitencourt Rosa, Duarte Marta Maria Medeiros Frescura, Vogel Fernanda Silveira Flores, de La Rue Mario Luiz, dos Anjos Lopes Sonia Terezinha
Department of Small Animal, Universidade Federal de Santa Maria, Brazil.
Korean J Parasitol. 2013 Aug;51(4):421-6. doi: 10.3347/kjp.2013.51.4.421. Epub 2013 Aug 30.
Several studies have shown the mechanisms and importance of immune responses against Toxoplasma gondii infection and the notable role of cholinesterases in inflammatory reactions. However, the association between those factors has not yet been investigated. Therefore, the aim of this study was to evaluate the acetylcholinesterase (AChE) activity in blood and lymphocytes and the activity of butyrylcholinesterase (BChE) in serum of rats experimentally infected with T. gondii during the acute phase of infection. For that, an in vivo study was performed with evaluations of AChE and BChE activities on days 5 and 10 post-infection (PI). The activity of AChE in blood was increased on day 5 PI, while in lymphocytes its activity was enhanced on days 5 and 10 PI (P<0.05). No significant difference was observed between groups regarding to the activity of BChE in serum. A positive (P<0.01) correlation was observed between AChE activity and number of lymphocytes. The role of AChE as an inflammatory marker is well known in different pathologies; thus, our results lead to the hypothesis that AChE has an important role in modulation of early immune responses against T. gondii infection.
多项研究表明了针对弓形虫感染的免疫反应机制及重要性，以及胆碱酯酶在炎症反应中的显著作用。然而，这些因素之间的关联尚未得到研究。因此，本研究的目的是评估实验性感染弓形虫的大鼠在感染急性期血液中的乙酰胆碱酯酶（AChE）活性和淋巴细胞中的AChE活性，以及血清中的丁酰胆碱酯酶（BChE）活性。为此，进行了一项体内研究，在感染后第5天和第10天评估AChE和BChE活性。感染后第5天血液中AChE活性增加，而淋巴细胞中其活性在感染后第5天和第10天增强（P<0.05）。各组血清中BChE活性未见显著差异。观察到AChE活性与淋巴细胞数量之间呈正相关（P<0.01）。AChE作为炎症标志物在不同病理情况下的作用是众所周知的；因此，我们的结果得出这样的假设，即AChE在调节针对弓形虫感染的早期免疫反应中起重要作用。
