新型 FeO-CaAl 层状双氢氧化物-左旋多巴磁响应性药物输送系统对黑素瘤癌细胞的抗增殖作用。
Antiproliferative effects of new magnetic pH-responsive drug delivery system composed of FeO, CaAl layered double hydroxide and levodopa on melanoma cancer cells.
机构信息
Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran; Medical Biology Research Center (MBRC), University of Medical Sciences, Kermanshah, Iran.
Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran.
出版信息
Mater Sci Eng C Mater Biol Appl. 2019 Aug;101:472-486. doi: 10.1016/j.msec.2019.04.004. Epub 2019 Apr 4.
In this study, an efficient drug delivery system composed of FeO, CaAl layered double hydroxide (LDH) and l-Dopa has been synthesized through hydrogen bonds between l-Dopa and CaAl-LDH encapsulated FeO nanoparticles (FeO@CaAl-LDH@l-Dopa). The structural features of FeO@CaAl-LDH@l-Dopa were characterized using XRD, SEM, TEM, EDX, FT-IR, VSM, TGA, XPS, zeta potential analysis and BET. All of the characterization techniques show the uniform high surface area core-shell structure with about 120 nm in average size. Also, the obtained results clearly indicate that this drug delivery system possess high potent for adsorption of l-Dopa (52 wt%) and high drug encapsulation efficiency (71%). The amount of l-Dopa release in low pHs (53.8%) which simulates the environment of cancer cells is greater than higher pHs. The in vitro cytotoxic and anticancer activities of FeO@CaAl-LDH@l-Dopa were investigated against Mel-Rm Cells Melanoma (NCIt: C3224) using LDH colorimetric assay and differential staining cell death assay. The results showed FeO@CaAl-LDH@l-Dopa with a lower concentration of l-Dopa, illustrate a higher cytotoxicity and anticancer activity.
在这项研究中，通过 l-Dopa 与包裹在 FeO 纳米颗粒中的 CaAl 层状双氢氧化物（LDH）之间的氢键，合成了由 FeO、CaAl 层状双氢氧化物（LDH）和 l-Dopa 组成的高效药物输送系统（FeO@CaAl-LDH@l-Dopa）。使用 XRD、SEM、TEM、EDX、FT-IR、VSM、TGA、XPS、zeta 电位分析和 BET 对 FeO@CaAl-LDH@l-Dopa 的结构特征进行了表征。所有的表征技术都表明该药物输送系统具有均匀的高表面积核壳结构，平均粒径约为 120nm。此外，所得结果清楚地表明，该药物输送系统具有高吸附 l-Dopa（52wt%）和高药物包封效率（71%）的能力。在模拟癌细胞环境的低 pH 值（53.8%）下，l-Dopa 的释放量大于较高 pH 值下的释放量。使用 LDH 比色法和差示染色细胞死亡法，对 FeO@CaAl-LDH@l-Dopa 对 Mel-Rm 细胞（黑色素瘤，NCIt：C3224）的体外细胞毒性和抗癌活性进行了研究。结果表明，FeO@CaAl-LDH@l-Dopa 以较低浓度的 l-Dopa 表现出更高的细胞毒性和抗癌活性。
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