School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
Mater Sci Eng C Mater Biol Appl. 2019 Aug;101:619-629. doi: 10.1016/j.msec.2019.04.012. Epub 2019 Apr 9.
In order to broaden the abilities of injectable hydrogel scaffolds, a self-healing chitosan/alginate hydrogel encapsulated with magnetic gelatin microspheres (MGMs) was prepared for anti-cancer drug delivery and soft tissue engineering. The hydrogel was formulated by cross-linking carboxyethyl chitosan (CEC) and oxidized alginate (OAlg) via the Schiff-base reaction. To strengthen the mechanical and biological capabilities of hydrogel, MGMs containing 5-fluorouracil (5-Fu) were prepared by an emulsion cross-linking method. In vitro gelation time, swelling ratio, degradation, compressive modulus and rheological behaviors were tested to monitor the effect of MGMs on the CEC-OAlg hydrogel. With a concentration of 30 mg/mL MGMs, the composite hydrogel provided with the suitable performance and showed excellent self-healing ability under physiological condition. Moreover, this composite hydrogel showed the sustained in vitro drug release compared with control MGMs and CEC-OAlg hydrogel. Our results demonstrated that this magnetic and self-healing CEC-OAlg hydrogel scaffold encapsulated MGMs containing 5-Fu was expected to be a platform for drug delivery and soft tissue engineering.
为了拓宽可注射水凝胶支架的功能，制备了一种具有自修复能力的壳聚糖/海藻酸钠水凝胶，用于载药和软组织工程。该水凝胶通过席夫碱反应交联羧乙基壳聚糖（CEC）和氧化海藻酸钠（OAlg）制备。为了增强水凝胶的机械和生物学性能，通过乳液交联法制备了载有 5-氟尿嘧啶（5-Fu）的磁性明胶微球（MGMs）。通过体外凝胶时间、溶胀比、降解、压缩模量和流变行为测试来监测 MGMs 对 CEC-OAlg 水凝胶的影响。在 MGMs 浓度为 30mg/mL 时，复合水凝胶具有合适的性能，并在生理条件下表现出优异的自修复能力。此外，与对照 MGMs 和 CEC-OAlg 水凝胶相比，该复合水凝胶表现出持续的体外药物释放。我们的结果表明，这种载有 5-Fu 的磁性和自修复 CEC-OAlg 水凝胶支架有望成为药物输送和软组织工程的平台。
