Ling Sumei, Li Xiulan, Zhang Danping, Wang Ke, Zhao Wenwen, Zhao Qiang, Wang Rongzhi, Yuan Jun, Xin Sijie, Wang Shihua
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Toxicon. 2019 Jul;165:103-109. doi: 10.1016/j.toxicon.2019.04.011. Epub 2019 Apr 25.
Tetrodotoxin (TTX) is a neurotoxin mainly responsible for severe neurological illness, and okadaic acid (OA) is another important lipophilic toxin to humans. In this study, we developed a gold strip for simultaneous detection of OA and TTX in real seafood samples. In the assay, the prepared nanoparticles (about 40 nm) was applied to conjugate with specific monoclonal antibodies against OA and TTX, and the resulted mixtures were used to capture its corresponding toxin in test strip. OA and TTX conjugates were coated as two test lines on the nitrocellulose membrane, and goat anti-mouse IgG was used to form the control line, forming three lines on the test strip. The visual detection limits (vLOD) of this immunoassay for OA and TTX were 0.75 and 15 ng/mL, respectively, and no cross reactions were observed in the process of detection. The visual assay for OA and TTX detection could be finished within 10 min. This study might provide a feasible method and good understanding for rapidly simultaneous detection for toxins based on immunoassay.
河豚毒素（TTX）是一种主要导致严重神经疾病的神经毒素，而冈田酸（OA）是另一种对人类重要的亲脂性毒素。在本研究中，我们开发了一种用于同时检测实际海鲜样品中OA和TTX的金标试纸条。在该检测方法中，将制备的纳米颗粒（约40纳米）与抗OA和TTX的特异性单克隆抗体偶联，所得混合物用于在试纸条中捕获其相应毒素。OA和TTX偶联物作为两条检测线包被在硝酸纤维素膜上，用羊抗鼠IgG形成对照线，在试纸条上形成三条线。该免疫分析法对OA和TTX的目视检测限（vLOD）分别为0.75和15纳克/毫升，且在检测过程中未观察到交叉反应。对OA和TTX检测的目视分析可在10分钟内完成。本研究可能为基于免疫分析法快速同时检测毒素提供一种可行的方法和良好的理解。
