Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Faculty of Medicine, Medical Center-University of Freiburg, University of Freiburg, Freiburg, Germany.
Department of Orthodontics, Center for Dental Medicine, Faculty of Medicine, Medical Center-University of Freiburg, University of Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany.
Clin Oral Investig. 2020 Jan;24(1):113-122. doi: 10.1007/s00784-019-02908-x. Epub 2019 Apr 27.
The aim of the present study was to evaluate the growth and acid synthesis of cariogenic pathogens in vitro when rebaudioside A was the sole source of carbon, compared to sucrose, xylitol, and a commercial sweetener containing rebaudioside A.
First, sucrose or sugar substitutes were added to human saliva and the pH was measured over 10 h. Subsequently, the growth and acid synthesis efficiency of Streptococcus mutans, Streptococcus sobrinus, Streptococcus oralis, Lactobacillus rhamnosus, Lactobacillus paracasei, and Candida albicans were investigated: after adding them to minimal culture media containing only one of the carbon sources, the optical density and pH were determined over 10 h.
A significant pH drop only occurred in the sucrose group of human saliva whereas no differences were found between the sugar substitute groups. The individual strains of caries pathogens showed significantly lower growth rates and less acid synthesis in the sugar replacement groups, in comparison to sucrose. However, these groups also showed bacterial growth and a decline in pH.
Xylitol appeared to be superior to rebaudioside A regarding the inhibition of mutans streptococci. The results for the remaining microorganisms indicate that rebaudioside A causes a similar or lower acid synthesis than xylitol. As saliva contains buffer systems, the metabolism of rebaudioside A did not seem to shift the equilibrium to demineralization.
Rebaudioside A may not be suitable as a targeted Streptococcus mutans treatment, but it could contribute to a reduction of caries risk as a replacement for sucrose.
本研究旨在评估以莱鲍迪甙 A 为唯一碳源时，与蔗糖、木糖醇和含有莱鲍迪甙 A 的商业甜味剂相比，致龋病原菌的生长和产酸情况。
首先，在人唾液中添加蔗糖或糖替代品，并在 10 小时内测量 pH 值。随后，研究了变异链球菌、远缘链球菌、口腔链球菌、鼠李糖乳杆菌、副干酪乳杆菌和白色念珠菌的生长和产酸效率：在向仅含有一种碳源的最小培养基中添加它们后，在 10 小时内测定光密度和 pH 值。
人唾液中的蔗糖组 pH 值明显下降，而糖替代品组之间无差异。与蔗糖相比，各致龋病原菌的生长速度和产酸量在糖替代组中明显较低。然而，这些组也显示出细菌生长和 pH 值下降。
木糖醇在抑制变形链球菌方面似乎优于莱鲍迪甙 A。对于其余微生物的结果表明，莱鲍迪甙 A 引起的产酸量与木糖醇相似或更低。由于唾液中含有缓冲系统，莱鲍迪甙 A 的代谢似乎不会使平衡向脱矿质方向移动。
莱鲍迪甙 A 可能不适合作为靶向变异链球菌的治疗方法，但它可以作为蔗糖的替代品，有助于降低龋齿风险。
