Ajagannanavar Sunil Lingaraj, Battur Hemant, Shamarao Supreetha, Sivakumar Vivek, Patil Pavan Uday, Shanavas P
Senior Lecturer, Department of Public Health Dentistry, KVG Dental College & Hospital, Sullia, Dakshina Kannada, Karnataka, India.
Reader, Department of Public Health Dentistry, KVG Dental College & Hospital, Sullia, Dakshina Kannada, Karnataka, India.
J Int Oral Health. 2014 Jul;6(4):29-34.
Glycyrrhiza (licorice) an herb, which has medicinal value and was used in ancient times as a remedy for a great diversity of ailments and sweetener. Roots of Glycyrrhiza contain a high concentration of saponin and glycyrrhizin, which are supposed to be sweetening agents. The aim of the study was to compare the efficacy of aqueous and alcoholic licorice root extract against Streptococcus mutans and Lactobacillus acidophilus in comparison to chlorhexidine (CHX).
In the first part of the study, various concentrations of aqueous and ethanolic licorice extract were prepared in the laboratory of Pharmacy College. It was then subjected to microbiological assay to determine its zone of inhibition using agar disk diffusion test and minimum inhibitory concentration (MIC) using serial broth dilution method against S. mutans and L. acidophilus. CHX was used as a positive control.
MIC of aqueous and ethnolic licorice root extract against S. mutans and L. acidophilus were 25% and 12.5%, respectively. Mean zone of inhibition of the aqueous and alcoholic licorice extracts against S. mutans at 48 h were 22.8 mm and 26.7 mm, respectively. Mean zone of inhibition of the aqueous and alcoholic licorice extracts against L. acidophilus at 48 h were 14.4 mm and 15.1 mm, respectively. Mean zone of inhibition of the CHX against S. mutans and L. acidophilus at 48 h was 20.5 and 13.2, respectively.
The inhibitory effect shown by alcoholic licorice root extract against S. mutans and L. acidophilus was superior when compared with that of aqueous form and CHX.
甘草是一种具有药用价值的草药，在古代被用作治疗多种疾病的药物和甜味剂。甘草根含有高浓度的皂苷和甘草甜素，它们被认为是甜味剂。本研究的目的是比较甘草根水提取物和醇提取物与氯己定（CHX）相比对变形链球菌和嗜酸乳杆菌的疗效。
在研究的第一部分，在药学院实验室制备了不同浓度的甘草水提取物和乙醇提取物。然后对其进行微生物学测定，使用琼脂纸片扩散试验确定其抑菌圈，并使用系列肉汤稀释法测定其对变形链球菌和嗜酸乳杆菌的最低抑菌浓度（MIC）。CHX用作阳性对照。
甘草根水提取物和乙醇提取物对变形链球菌和嗜酸乳杆菌的MIC分别为25%和12.5%。48小时时，甘草水提取物和醇提取物对变形链球菌的平均抑菌圈分别为22.8毫米和26.7毫米。48小时时，甘草水提取物和醇提取物对嗜酸乳杆菌的平均抑菌圈分别为14.4毫米和15.1毫米。48小时时，CHX对变形链球菌和嗜酸乳杆菌的平均抑菌圈分别为20.5和13.2。
与甘草根水提取物和CHX相比，甘草根醇提取物对变形链球菌和嗜酸乳杆菌的抑制作用更强。
