Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland.
Department of Medical Microbiology, University Medical Center, Hospital of the Infant Jesus, Warsaw, Poland.
Eur J Clin Microbiol Infect Dis. 2019 Jul;38(7):1339-1342. doi: 10.1007/s10096-019-03565-4. Epub 2019 Apr 27.
Corynebacterium coyleae is part of the commensal microflora of the skin, urethra, mucous membranes, and genital tract. Isolates from patients with urinary tract infection (UTI) were reported, but the pathogenic potential of this species has not been defined yet. The aim of the study is to determine whether C. coyleae could be the etiological agent of UTI and to analyze its antibiotic susceptibility. Urine samples were cultured quantitatively according to accepted laboratory procedures. The identification of bacterial isolates was carried out using the Vitek MS (bioMérieux) and antibiotic susceptibility was tested using disc diffusion according to EUCAST guidelines. Between 1 January 2017 and 30 October 2018, a total of 39 C. coyleae strains were isolated. This represented 0.32% of all urine samples cultured in the laboratory during the collection period. The strains were isolated from samples obtained from 35 women and 3 men (age median for all-64 years). One female patient presented with C. coyleae in her urine twice at an interval of 21 months. In six cases of UTI, C. coyleae was isolated in monoculture. The isolates had the same resistance pattern. A total of 11 strains were obtained from cases with a clinical diagnosis of UTI. In 13 cases, the strain was cultured in a monoculture and in 28 cases with accompanying species. All strains were susceptible to vancomycin. However, resistance to ciprofloxacin was observed for 58.4% of the strains. Urine isolates of C. coyleae must be considered as contamination or normal flora in most cases (28/39, 72%). In the remaining cases, it can be considered as potential etiologic agents, mostly in women and especially in the 6 UTI cases where C. coyleae was found as the single culture-positive species. Several of these isolates demonstrate resistance to antibiotics commonly used in empiric treatment of urinary tract infections.
科氏棒杆菌是皮肤、尿道、黏膜和生殖道共生微生物群的一部分。已报道从尿路感染（UTI）患者中分离出该菌，但该物种的致病潜力尚未确定。本研究旨在确定科氏棒杆菌是否可能是 UTI 的病原体，并分析其抗生素敏感性。尿液样本按照公认的实验室程序进行定量培养。使用 Vitek MS（生物梅里埃）进行细菌分离物的鉴定，并根据 EUCAST 指南使用纸片扩散法测试抗生素敏感性。2017 年 1 月 1 日至 2018 年 10 月 30 日，共分离出 39 株科氏棒杆菌。这占实验室培养的所有尿液样本的 0.32%。在收集期间，这些菌株从 35 名女性和 3 名男性的样本中分离出来（所有人的年龄中位数为 64 岁）。一名女性患者在 21 个月的间隔内两次尿液中分离出科氏棒杆菌。在 6 例 UTI 中，科氏棒杆菌在单一培养物中分离出来。分离株具有相同的耐药模式。总共从临床诊断为 UTI 的 11 例中获得 11 株。在 13 例中，菌株在单一培养物中培养，在 28 例中与伴随物种一起培养。所有菌株对万古霉素均敏感。然而，58.4%的菌株对环丙沙星表现出耐药性。在大多数情况下（28/39，72%），尿液分离的科氏棒杆菌必须被视为污染或正常菌群。在其余情况下，它可以被认为是潜在的病原体，尤其是在科氏棒杆菌作为唯一培养阳性物种的 6 例 UTI 中。这些分离株中的几个对常用于经验性治疗尿路感染的抗生素表现出耐药性。
