Anwar Ayaz, Siddiqui Ruqaiyyah, Raza Shah Muhammad, Khan Naveed Ahmed
Department of Biological Sciences, School of Science and Technology, Sunway University, Selangor, 47500, Malaysia.
HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan.
J Microbiol Biotechnol. 2019 May 28;29(5):713-720. doi: 10.4014/jmb/1903.03009.
belonging to the T4 genotype may cause a fatal brain infection known as granulomatous amoebic encephalitis, and the vision-threatening eye infection keratitis. The aim of this study was to evaluate the antiamoebic effects of three clinically available antidiabetic drugs, Glimepiride, Vildagliptin and Repaglinide, against belonging to the T4 genotype. Furthermore, we attempted to conjugate these drugs with silver nanoparticles (AgNPs) to enhance their antiamoebic effects. Amoebicidal, encystation, excystation, and host cell cytotoxicity assays were performed to unravel any antiacanthamoebic effects. Vildagliptin conjugated silver nanoparticles (Vgt-AgNPs) characterized by spectroscopic techniques and atomic force microscopy were synthesized. All three drugs showed antiamoebic effects against and significantly blocked the encystation. These drugs also showed significant cysticidal effects and reduced host cell cytotoxicity caused by . Moreover, Vildagliptin-coated silver nanoparticles were successfully synthesized and are shown to enhance its antiacanthamoebic potency at significantly reduced concentration. The repurposed application of the tested antidiabetic drugs and their nanoparticles against free-living amoeba such as described here is a novel outcome that holds tremendous potential for future applications against devastating infection.
属于T4基因型的（某种生物）可能会引发一种名为肉芽肿性阿米巴脑炎的致命脑部感染，以及威胁视力的眼部感染——角膜炎。本研究的目的是评估三种临床可用的抗糖尿病药物格列美脲、维格列汀和瑞格列奈对属于T4基因型的（某种生物）的抗阿米巴作用。此外，我们尝试将这些药物与银纳米颗粒（AgNPs）结合，以增强它们的抗阿米巴作用。进行了杀阿米巴、包囊形成、脱囊和宿主细胞细胞毒性试验，以揭示任何抗棘阿米巴作用。合成了通过光谱技术和原子力显微镜表征的维格列汀缀合银纳米颗粒（Vgt-AgNPs）。所有三种药物均显示出对（某种生物）的抗阿米巴作用，并显著阻断了包囊形成。这些药物还显示出显著的杀囊作用，并降低了由（某种生物）引起的宿主细胞细胞毒性。此外，成功合成了维格列汀包被的银纳米颗粒，并显示出在显著降低的浓度下增强其抗棘阿米巴效力。本文所述的受试抗糖尿病药物及其纳米颗粒对诸如（某种生物）等自由生活阿米巴的重新利用是一个新成果，对未来对抗毁灭性感染具有巨大的应用潜力。
