来自古细菌tRNA的一种新型荧光三环核苷的结构测定
Structure determination of a new fluorescent tricyclic nucleoside from archaebacterial tRNA.
作者信息
McCloskey J A, Crain P F, Edmonds C G, Gupta R, Hashizume T, Phillipson D W, Stetter K O
出版信息
Nucleic Acids Res. 1987 Jan 26;15(2):683-93. doi: 10.1093/nar/15.2.683.
Abstract
A highly fluorescent nucleoside was detected in enzymatic digests of the extremely thermophilic archaebacterium Sulfolobus solfataricus by combined liquid chromatography-mass spectrometry (LC/MS). Following isolation, the structure was determined primarily by mass spectrometry, to be 3-(beta-D-ribofuranosyl)-4,9-dihydro-4,6,7-trimethyl-9-oxoimidazo[ 1, 2-a]purine (mimG), a new derivative of the Y (wye) nucleoside. The structural assignment was verified by comparison of the base released by acid hydrolysis with the corresponding synthetic base, using mass spectrometry, chromatography, and UV absorption and fluorescence properties. Nucleoside mimG was also detected by LC/MS in hydrolysates of the thermophiles Thermoproteus neutrophilus and Pyrodictium occultum. These results constitute the first finding of a member of the hypermodified Y family of nucleosides in archaebacteria.
摘要
通过液相色谱 - 质谱联用（LC/MS）在嗜热古细菌嗜热栖热菌的酶解产物中检测到一种高荧光核苷。分离后，主要通过质谱确定其结构为3 - （β - D - 呋喃核糖基）- 4,9 - 二氢 - 4,6,7 - 三甲基 - 9 - 氧代咪唑并[1,2 - a]嘌呤（mimG），一种新型的Y（怀依）核苷衍生物。通过质谱、色谱以及紫外吸收和荧光特性，将酸水解释放的碱基与相应的合成碱基进行比较，验证了结构归属。通过LC/MS在嗜热栖热菌和隐蔽火球菌的水解产物中也检测到了核苷mimG。这些结果构成了在古细菌中首次发现超修饰Y家族核苷成员的发现。
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