State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Lab Chip. 2019 May 14;19(10):1783-1789. doi: 10.1039/c9lc00223e.
The ultrasensitive detection of multiple nucleic acids has great significance in a broad range of medical fields since nucleic acid samples are usually of small volumes and highly diluted. Herein, we present a novel magnetic photonic crystal (PhC) barcode-integrated condensing-enriched superhydrophobic platform for the ultrasensitive multiple miRNA detection. Droplets containing targets could suck the hydrogel PhC barcodes without losing their targets due to the difference in wettability between the hydrophilic PhC barcodes and hydrophobic substrate. During the evaporation of water from the droplet microreactors, these targets could be effectively enriched in the barcodes for achieving higher sensitivities. As the encoding signals of the PhC barcodes are the characteristic reflection peaks generated by their ordered physical nanostructures, the barcodes are stable and free from any fluorescent background and photobleaching; thus, they are accurate for distinguishing different targets. In addition, with the integration of magnetic nanoparticles into the hydrogel PhC barcodes, they could be imparted with the features of immobilization during change of medium and flexible movement for controlling the reaction, both of which could facilitate the detection processes. Based on this platform, we have demonstrated that the detection limit of multiple miRNAs is improved by about three orders. Thus, our platform is an ideal alternative for the ultrasensitive simultaneous multiplex analysis in medical fields.
由于核酸样本体积小且高度稀释，因此对多种核酸的超灵敏检测在广泛的医学领域具有重要意义。在这里，我们提出了一种新颖的磁性光子晶体（PhC）条码集成凝聚富集超疏水平台，用于超灵敏的多种 miRNA 检测。由于亲水 PhC 条码和疏水基底之间的润湿性差异，含有靶标的液滴可以吸取水凝胶 PhC 条码而不会丢失其靶标。在液滴微反应器中的水蒸发过程中，这些靶标可以有效地富集在条码中，从而实现更高的灵敏度。由于 PhC 条码的编码信号是由其有序的物理纳米结构产生的特征反射峰，因此条码稳定且不受任何荧光背景和光漂白的影响；因此，它们可以准确地区分不同的靶标。此外，通过将磁性纳米粒子集成到水凝胶 PhC 条码中，它们可以在介质变化时赋予固定化的特性，并且可以灵活移动以控制反应，这两者都可以促进检测过程。基于该平台，我们已经证明，多种 miRNAs 的检测限提高了约三个数量级。因此，我们的平台是医学领域中用于超灵敏同时多重分析的理想替代方案。
