Department of Cardiology , Zhongda Hospital Affiliated with Southeast University , Nanjing , Jiangsu 210009 , China.
School of Engineering and Applied Sciences and Department of Physics , Harvard University , Cambridge , Massachusetts 02138 , United States.
ACS Sens. 2019 May 24;4(5):1384-1390. doi: 10.1021/acssensors.9b00352. Epub 2019 Apr 19.
Early detection of cardiac troponin I (cTnI), B-type natriuretic peptide (BNP), and myoglobin (Myo) is essential for the diagnosis of acute myocardial infarction (AMI) and heart failure (HF). We designed a porous hydrogel-encapsulated photonic crystal (PhC) barcode-based suspension array for multiple cardiovascular marker detection. The hybrid hydrogel was composed of polyethylene glycol diacrylate (PEGDA) and gelatin, resulting in a porous and hydrophilic scaffold which ensured stability of the PhC in aqueous solutions. The encapsulated PhC barcodes had stable diffraction peaks for the corresponding markers. Using a sandwich format, the proposed suspension array was used for simultaneous multiplex detection of cardiovascular biomarkers in a single tube. The immunoassay results we tested on cTnI, BNP, and Myo could be assayed in the ranges of 0.01 to 1000 ng/mL, 0.1 to 10 000 pg/mL, and 1 to 10 000 ng/mL with limits of detection of 0.009 ng/mL, 0.084 pg/mL, and 0.68 ng/mL at 3σ, respectively. This method also showed acceptable accuracy and repeated detection, and the results were consistent with the results of conventional clinical methods for detecting actual clinical samples. Therefore, suspension arrays based on hydrogel-encapsulated PhC barcodes are highly promising for AMI diagnosis.
早期检测心肌肌钙蛋白 I（cTnI）、B 型利钠肽（BNP）和肌红蛋白（Myo）对于急性心肌梗死（AMI）和心力衰竭（HF）的诊断至关重要。我们设计了一种基于多孔水凝胶包封的光子晶体（PhC）条码的悬浮阵列，用于多种心血管标志物的检测。该混合水凝胶由聚乙二醇二丙烯酸酯（PEGDA）和明胶组成，形成多孔亲水支架，确保 PhC 在水溶液中的稳定性。包封的 PhC 条码具有相应标志物的稳定衍射峰。采用夹心模式，该悬浮阵列可在单个管中同时进行多种心血管生物标志物的多重检测。我们在 cTnI、BNP 和 Myo 上测试的免疫分析结果可在 0.01 至 1000 ng/mL、0.1 至 10000 pg/mL 和 1 至 10000 ng/mL 的范围内进行测定，检测限分别为 0.009 ng/mL、0.084 pg/mL 和 0.68 ng/mL（3σ）。该方法还表现出可接受的准确性和重复性检测，并且与实际临床样本的常规临床方法的检测结果一致。因此，基于水凝胶包封的 PhC 条码的悬浮阵列非常有希望用于 AMI 诊断。
