GREENTECH SA, Biopôle Clermont-Limagne, 63360, Saint-Beauzire, France.
Deinove, 1682 rue de la Valsiere, 34790, Grabels, France.
Int J Cosmet Sci. 2019 Aug;41(4):391-397. doi: 10.1111/ics.12534. Epub 2019 Jun 17.
Intrinsic skin ageing is mainly caused by cellular senescence. p16 and p21 are two important tumour suppressor proteins that play essential roles during cell proliferation and ageing through regulating the expression of several genes. Moreover, physical changes between the ages of 55 and 60 years affect one's physical and disrupt self-esteem. The cosmetics industry has focused on bioactive substances derived from natural products such as plants, mushrooms and marine algae to counteract the deleterious effect on skin senescence. Besides these products, compounds produced by bacteria may decelerate individual senescence.
In order to evaluate the potential benefits of bacteria extract over skin ageing, we investigated whether a Sphingomonas hydrophobicum (SH) extract is able to protect our skin against senescence mechanisms. We used an ageing full-thickness skin equivalent model, which was treated or not with the bacteria extract in a systemic way for 42 days. p21 and p16 and senescence-associated galactosidase activity were used to detect cellular senescence with immunohistology. Using a psychobiological approach, we evaluated in vivo the effect of SH extract on self-esteem, isotropy and suppleness.
Sphingomonas extract significantly suppressed senescence associated with β-galactosidase activation. It also significantly inhibited the expression of cell cycle inhibitors (p21 and p16). At the same time, the bacteria extract has a significant positive impact on the issue by increasing the expression of versican and fibrillin-1. Significant improvements of self-esteem were reported after 56 days of SH extract application. These psychological benefits were accompanied by a significant improvement in skin suppleness and isotropy.
Sphingomonas extract delays intrinsic skin ageing process by inhibiting cellular senescence, and has also the capability to restructure the skin. These beneficial physiological effects induced by SH extract have a positive influence on self-esteem. Because skin ageing causes emotional distress, SH extract can serve as an anti-ageing cosmeceutical agent and help to build a better psychological health, and help individuals to assume ageing.
内在皮肤老化主要由细胞衰老引起。p16 和 p21 是两种重要的肿瘤抑制蛋白，通过调节几个基因的表达，在细胞增殖和衰老过程中发挥着重要作用。此外，55 岁至 60 岁之间的身体变化会影响身体外观并破坏自尊心。化妆品行业一直专注于从植物、蘑菇和海洋藻类等天然产物中提取生物活性物质，以对抗皮肤衰老的有害影响。除了这些产品，细菌产生的化合物可能会减缓个体衰老。
为了评估细菌提取物对皮肤衰老的潜在益处，我们研究了 Sphingomonas hydrophobicum (SH) 提取物是否能够保护我们的皮肤免受衰老机制的影响。我们使用了一种老化的全层皮肤等效模型，该模型以全身性方式用或不用细菌提取物处理 42 天。使用免疫组织化学法检测细胞衰老，检测 p21 和 p16 和衰老相关的半乳糖苷酶活性。采用心理生物学方法，我们评估了 SH 提取物对自尊、各向同性和柔韧性的体内影响。
Sphingomonas 提取物显著抑制了与β-半乳糖苷酶激活相关的衰老。它还显著抑制了细胞周期抑制剂（p21 和 p16）的表达。同时，细菌提取物通过增加 versican 和纤维蛋白-1 的表达对该问题有显著的积极影响。在应用 SH 提取物 56 天后，报告了自尊的显著提高。这些心理益处伴随着皮肤柔韧性和各向同性的显著改善。
Sphingomonas 提取物通过抑制细胞衰老来延缓内在皮肤衰老过程，并且还具有重塑皮肤的能力。SH 提取物引起的这些有益的生理效应对自尊产生积极影响。由于皮肤衰老会引起情绪困扰，因此 SH 提取物可用作抗衰老化妆品，有助于建立更好的心理健康，并帮助个人接受衰老。
