Graduate Program in Dentistry, Federal University of Pelotas, Pelotas, Brazil.
Department of Stomatology, School of Dentistry, Federal University of Santa Maria, Santa Maria, Brazil.
Int Endod J. 2019 Oct;52(10):1446-1456. doi: 10.1111/iej.13135. Epub 2019 May 15.
To evaluate bone tissue reactions in rats to an MTA-based endodontic sealer with and without the addition of various concentrations of C3A or C3A + Ag.
Bone tissue reactions were evaluated in 45 Wistar rats after 7, 30 and 90 days (n = 5 per period). Three surgical cavities were prepared on the right femur and filled with 0.2 mL MTA Fillapex, MTA Fillapex + C3A and C3A + Ag at various concentrations: AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany), EndoSequence BC (Brasseler USA, Savannah, GA, USA) or no sealer (negative control). By the end of each experimental period, animals were randomly euthanized. The samples were histologically processed and analysed using a light microscope. The presence of inflammatory cells, fibres and hard tissue barrier formation was evaluated. Data were analysed statistically using nonparametric tests to compare the differences between groups. Multiple groups were compared using the Kruskal-Wallis and Mann-Whitney U-tests with a Bonferroni correction at P = 0.05.
The inflammatory response significantly decreased from 30 to 90 days (P < 0.05). Fibre condensation was similar amongst the groups at 07 and 30 days after intervention (P > 0.05). At 90 days, however, fibres were absent in most specimens of EndoSequence BC Sealer, AH Plus, MTA Fillapex and the control group, whilst they were still observed in samples of the modified sealers (P < 0.05). At 90 days, all specimens of AH Plus, EndoSequence BC Sealer and control group had complete formation of hard tissue barrier. In the MTA Fillapex group, as well as in the modified sealers groups, partial deposition of mineralized tissue was noticed.
The hypothesis tested that the incorporation of C3A and C3A + Ag particles to MTA Fillapex would improve bone tissue repair was partially accepted, since modified MTA Fillapex did not have the same repair potential as the commercial bioceramic material.
评估含有不同浓度 C3A 或 C3A+Ag 的基于 MTA 的根管封闭剂在大鼠骨组织中的反应。
在 45 只 Wistar 大鼠的右侧股骨上制备 3 个手术腔，分别用 0.2 mL MTA Fillapex、MTA Fillapex+C3A 和 C3A+Ag 填充，浓度分别为 AH Plus（Dentsply DeTrey GmbH，德国康斯坦茨）、EndoSequence BC（Brasseler USA，美国佐治亚州萨凡纳）或无封闭剂（阴性对照）。在每个实验期结束时，随机处死动物。对样本进行组织学处理，用光学显微镜进行分析。评估炎症细胞、纤维和硬组织屏障形成的存在。使用非参数检验对数据进行统计学分析，以比较组间差异。使用 Kruskal-Wallis 和 Mann-Whitney U 检验比较多组数据，并使用 Bonferroni 校正进行 P<0.05 的多重比较。
炎症反应从第 30 天到第 90 天显著降低（P<0.05）。干预后 07 天和 30 天，各组纤维凝结相似（P>0.05）。然而，90 天时，EndoSequence BC 密封剂、AH Plus、MTA Fillapex 和对照组的大多数标本中纤维消失，而改良密封剂的标本中仍可见纤维（P<0.05）。90 天时，AH Plus、EndoSequence BC 密封剂和对照组的所有标本均形成完整的硬组织屏障。在 MTA Fillapex 组以及改良密封剂组中，注意到矿化组织的部分沉积。
该研究部分接受了将 C3A 和 C3A+Ag 颗粒掺入 MTA Fillapex 可改善骨组织修复的假设，因为改良的 MTA Fillapex 没有与商业生物陶瓷材料相同的修复潜力。
