Mestieri Letícia Boldrin, Zaccara Ivana Maria, Pinheiro Lucas Siqueira, Barletta Fernando Branco, Kopper Patrícia Maria Polli, Grecca Fabiana Soares
Departament of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
School of Dentistry, Lutheran University of Brazil - ULBRA, Porto Alegre, RS, Brazil.
Restor Dent Endod. 2019 Nov 15;45(1):e2. doi: 10.5395/rde.2020.45.e2. eCollection 2020 Feb.
This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus.
BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test ( < 0.05).
The MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control ( < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control ( > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer ( < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound ( < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex ( < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing ( > 0.05).
All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.
本研究旨在评估Endosequence生物陶瓷根管封闭剂（BC封闭剂）与MTA Fillapex和AH Plus相比的细胞活力和迁移能力。
将BC封闭剂、MTA Fillapex和AH Plus与培养基接触，以1:1、1:2和1:4的稀释度获得封闭剂提取物。接种3T3细胞并使其暴露于提取物中。分别通过3-(4,5-二甲基噻唑基)-2,5-二苯基溴化四氮唑（MTT）和划痕试验评估细胞活力和迁移能力。数据采用Kruskal-Wallis检验和Dunn检验进行分析（P<0.05）。
MTT试验显示，与对照组相比，AH Plus和MTA Fillapex在1:1稀释度时具有更大的细胞毒性（P<0.05）。在1:2和1:4稀释度时，所有封闭剂均与对照组相似（P>0.05），且MTA Fillapex的细胞毒性比BC封闭剂更大（P<0.05）。划痕试验表明伤口随时间持续闭合。在30小时时，对照组伤口闭合（P<0.05）。在36小时时，与AH Plus和MTA Fillapex相比，只有BC封闭剂出现伤口闭合（P<0.05）。在42小时时，AH Plus和MTA Fillapex显示伤口愈合（P>0.05）。
所有测试的封闭剂均显示出细胞活力，突出了BC封闭剂，其显示出增加的细胞迁移能力，表明与其他测试的封闭剂相比，这种封闭剂可能实现更好的组织修复。
