Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangdong Provincial Engineering and Technological Research Center for Drug Carrier Development, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, People's Republic of China.
Biomed Mater. 2019 May 28;14(4):045013. doi: 10.1088/1748-605X/ab1d9c.
Magnetic resonance imaging (MRI), as a diagnostic tool in tissue engineering, has received widespread attention because of its ability to consistently provide degradation and absorption of implants in vivo. For some specific human tissues and organs, such as nerves, muscles and myocardium, their regeneration requires tissue engineering scaffolds have a good electrical conductivity. Graphene oxide (GO) has been extensively studied as a conductive biomaterial having mechanical reinforcement. Based on the above, we propose an MRI conductive scaffold containing gelatin (Gel)/gelatin-polycaprolactone (Gel-PCL)/ultra-small paramagnetic iron oxide (USPIO)/graphene oxide (GO) (Gel/Gel-PCL/USPIO/GO). Their physical and chemical properties as well as biocompatibility are measured in vitro. The purpose of doping USPIO was developed for non-invasive monitoring of tissue engineered implants and tissue reconstruction. Functional modification of GO to match electrophysiological requirement. Co-culture with bone marrow mesenchymal stem cells showed good biocompatibility. Blood experiments have also demonstrated the feasibility of scaffolds as tissue engineered implants. The USPIO-labeled conductive scaffold, as an effective image-guided and electrically stimulating implant, appears to be a reconstruction platform for specific tissues and organs.
磁共振成像（MRI）作为组织工程中的一种诊断工具，由于其能够持续提供体内植入物的降解和吸收，因此受到了广泛关注。对于某些特定的人体组织和器官，如神经、肌肉和心肌，其再生需要组织工程支架具有良好的导电性。氧化石墨烯（GO）作为一种具有机械增强作用的导电生物材料已被广泛研究。基于上述情况，我们提出了一种含有明胶（Gel）/明胶-聚己内酯（Gel-PCL）/超小超顺磁性氧化铁（USPIO）/氧化石墨烯（GO）（Gel/Gel-PCL/USPIO/GO）的 MRI 导电支架。体外测量了它们的物理化学性质和生物相容性。掺杂 USPIO 的目的是开发用于组织工程植入物和组织重建的非侵入性监测。GO 的功能修饰以匹配电生理要求。与骨髓间充质干细胞共培养显示出良好的生物相容性。血液实验也证明了支架作为组织工程植入物的可行性。USPIO 标记的导电支架作为一种有效的图像引导和电刺激植入物，似乎是特定组织和器官的重建平台。
