Department of Chemistry, Netaji Subhas Institute of Technology, Dwarka Sector 3, University of Delhi, New Delhi, 110078, India.
Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, India.
Carbohydr Polym. 2021 May 1;259:117501. doi: 10.1016/j.carbpol.2020.117501. Epub 2020 Dec 14.
The study's purpose was to fabricate a 3-D porous scaffold, in which chitosan was coated onto the pore wall of polycaprolactone (PCL) scaffolds as a bioactive agent to maximize the cell recognition signals, to improve the osteoconductivity of the scaffolds. The pppporogen leaching technique has been modified and used in the fabrication process, comprising of the coating of chitosan over the porogen followed by transferring of coating to the pore wall of the PCL scaffold. The cytotoxicity and hemolysis results indicated chitosan's presence over the surface of the scaffold's pore walls has significantly enhanced its biocompatibility. Scaffolds coated with 2.5 %(w/v) chitosan shows 6.74 % increase in porosity and 207.96 % upsurge in mechanical strength, compared to PCL scaffolds. The Gene-expression also proves the study groups of scaffolds show the minimal osteogenic expression. Therefore, chitosan coating over the scaffold's pore wall's surface opens an unconventional approach for tissue engineering applications.
本研究的目的是制备一种 3D 多孔支架，将壳聚糖涂覆在聚己内酯（PCL）支架的孔壁上作为生物活性物质，以最大限度地提高细胞识别信号，提高支架的骨传导性。已经对多孔体制备技术进行了改进并应用于制备过程中，包括在成孔剂上涂覆壳聚糖，然后将涂层转移到 PCL 支架的孔壁上。细胞毒性和溶血结果表明，壳聚糖存在于支架孔壁表面，显著提高了其生物相容性。与 PCL 支架相比，涂覆 2.5%（w/v）壳聚糖的支架的孔隙率增加了 6.74%，机械强度增加了 207.96%。基因表达也证明了支架的研究组表现出最小的成骨表达。因此，壳聚糖涂覆在支架孔壁表面为组织工程应用开辟了一种非传统的方法。
