McCormick S P, Bhatnagar D, Lee L S
Appl Environ Microbiol. 1987 Jan;53(1):14-6. doi: 10.1128/aem.53.1.14-16.1987.
Wild-type Aspergillus parasiticus produces, in addition to the colorless aflatoxins, a number of pigmented secondary metabolites. Examination of these pigments demonstrated that a major component was an anthraquinone, averufanin. Radiolabeling studies with [14C]averufanin showed that 23% of the label was incorporated into aflatoxin B1 by the wild type and that 31% of the label was incorporated into O-methylsterigmatocystin by a non-aflatoxin-producing isolate. In similar studies with blocked mutants of A. parasiticus the 14C label from averufanin was accumulated in averufin (72%) and versicolorin A (54%) but not averantin. The results demonstrate that averufanin is a biosynthetic precursor of aflatoxin B1 between averantin and averufin.
野生型寄生曲霉除了产生无色黄曲霉毒素外，还产生多种有色次生代谢产物。对这些色素的检测表明，主要成分是一种蒽醌，即疣孢青霉醌。用[14C]疣孢青霉醌进行的放射性标记研究表明，野生型将23%的标记物掺入黄曲霉毒素B1中，而一个不产生黄曲霉毒素的分离株将31%的标记物掺入O-甲基柄曲霉素中。在对寄生曲霉阻断突变体的类似研究中，来自疣孢青霉醌的14C标记物积累在疣孢菌素（72%）和杂色曲霉素A（54%）中，但未积累在疣孢青霉醇中。结果表明，疣孢青霉醌是黄曲霉毒素B1在疣孢青霉醇和疣孢菌素之间的生物合成前体。
