Zamir L O, Hufford K D
Appl Environ Microbiol. 1981 Jul;42(1):168-73. doi: 10.1128/aem.42.1.168-173.1981.
Kinetic pulse-labeling of aflatoxin pathway compounds was carried out in Aspergillus parasiticus, beginning with radioactive acetate. Norsolorinic acid, averufin, versicolorin A, and sterigmatocystin (all known as compounds which can be incorporated into the aflatoxin molecule) were radiotraced to follow their order of appearance. Aflatoxin species B1, B2, G1, and G2 were included. Norsolorinic acid and averufin appeared as early transient intermediates followed in order by versicolorin A, aflatoxins, and sterigmatocystin. To date, a mutually confirming array of results has been obtained with established precursors in wild-type strains of A. parasiticus and A. versicolor (as well as with an aflatoxin pathway mutant of A. parasiticus), which together establish a practical methodology for recognition of new pathway intermediates. The kinetic of pulse-labeling for sterigmatocystin in relation to aflatoxins suggests that duel branchlets may exist to flatoxins; i.e., sterigmatocystin may not be an obligatory aflatoxin precursor.
从放射性乙酸盐开始，在寄生曲霉中对黄曲霉毒素途径化合物进行了动力学脉冲标记。对柄曲霉素、暗黄霉素、杂色曲霉素和柄曲菌素（所有这些都被认为是可掺入黄曲霉毒素分子的化合物）进行放射性追踪，以了解它们的出现顺序。黄曲霉毒素B1、B2、G1和G2也包括在内。柄曲霉素和暗黄霉素作为早期短暂中间体出现，随后依次是杂色曲霉素、黄曲霉毒素和柄曲菌素。迄今为止，在寄生曲霉和杂色曲霉的野生型菌株（以及寄生曲霉的黄曲霉毒素途径突变体）中，使用已确定的前体获得了一系列相互印证的结果，这些结果共同建立了一种识别新途径中间体的实用方法。柄曲菌素相对于黄曲霉毒素的脉冲标记动力学表明，黄曲霉毒素可能存在双分支；即柄曲菌素可能不是黄曲霉毒素的必需前体。
