Çağlayan Kadriye, Roumi Vahid, Gazel Mona, Elçi Eminur, Acioğlu Mehtap, Mavric Plesko Irena, Reynard Jean-Sebastien, Maclot Francois, Massart Sebastien
Plant Protection Department, Agriculture Faculty, Mustafa Kemal University, 31034 Hatay, Turkey.
Plant Protection Department, Faculty of Agriculture, University of Maragheh, 55181 Maragheh, Iran.
Pathogens. 2019 Apr 27;8(2):57. doi: 10.3390/pathogens8020057.
High throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree ( cv. 0900 Ziraat) from Turkey identified a new member of the genus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5' UTR and 3' UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, , , , and share amino acid identities ranging from 43-53%, 44-60%, 39-43%, 38-44% and 45-50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies.
对从土耳其一棵甜樱桃树（品种0900 Ziraat）有症状叶片中分离的总RNA进行高通量测序，鉴定出一种新的病毒，命名为土耳其樱桃病毒（CVTR）。通过电子显微镜和重叠RT-PCR对其全基因组进行测序，证实了该病毒的存在。该病毒具有一个8464个核苷酸的单链RNA基因组，编码五个开放阅读框（ORF），并包含两个非编码区，5'UTR和3'UTR分别为97和296个核苷酸。与五种亲缘关系最近的罗比病毒相比，[此处应补充五种病毒名称]分别具有43%-53%、44%-60%、39%-43%、38%-44%和45%-50%的氨基酸同一性。与四种樱桃罗比病毒不同，CVTR缺乏ORF 2a和5a。因此，其基因组结构与非洲油棕环斑病毒（AOPRV）更相似。使用特异性引物，在从土耳其三个地区采集的156个测试样品中的15个甜樱桃和2个酸樱桃中证实了CVTR的存在。其中，五个样品的叶片出现轻微褪绿症状。似乎CVTR感染樱桃树时可能引发或不引发明显症状，但这些数据应在未来研究中通过在木本和可能的草本寄主上进行生物测定来证实。
