培养大鼠肝细胞中细胞色素P-450和混合功能氧化酶活性维持的品系差异。前列腺素的作用。
Strain differences in the maintenance of cytochrome P-450 and mixed-function-oxidase activities in cultured rat hepatocytes. Effect of prostaglandins.
作者信息
Grant M H, Smith S J, Burke M D
出版信息
Biochem J. 1986 Nov 1;239(3):785-8. doi: 10.1042/bj2390785.
The mixed-function-oxidase (MFO) activities, ethoxyresorufin and pentoxyphenoxazone O-dealkylase, of cultured Hooded-Lister(HL)-rat hepatocytes declined rapidly during 72 h of culture, whereas in Sprague-Dawley(SD)-rat hepatocytes the MFO activities increased between 24 and 72 h in culture. Cytochrome P-450 content declined at the same rate in both HL- and SD-rat hepatocyte cultures. NADPH:cytochrome c reductase and NADH:cytochrome b5 reductase were more stable in SD- than in HL-rat hepatocyte cultures. 16,16-Dimethylprostaglandins E2 and F2 alpha improved the maintenance of cytochrome P-450 content, MFO activity and NADPH:cytochrome c reductase in the HL-rat hepatocyte cultures. In SD-rat hepatocytes, the prostaglandins had no effect on cytochrome P-450 content or NADPH:cytochrome c reductase activity, whereas they prevented the increase observed in MFO activities between 24 and 72 h after culture.
培养的 Hooded-Lister（HL）大鼠肝细胞的混合功能氧化酶（MFO）活性，即乙氧异羟肟酸和戊氧基吩恶嗪 O-脱烷基酶，在培养 72 小时期间迅速下降，而在 Sprague-Dawley（SD）大鼠肝细胞中，MFO 活性在培养 24 至 72 小时之间增加。细胞色素 P-450 含量在 HL 和 SD 大鼠肝细胞培养物中以相同速率下降。NADPH：细胞色素 c 还原酶和 NADH：细胞色素 b5 还原酶在 SD 大鼠肝细胞培养物中比在 HL 大鼠肝细胞培养物中更稳定。16,16-二甲基前列腺素 E2 和 F2α改善了 HL 大鼠肝细胞培养物中细胞色素 P-450 含量、MFO 活性和 NADPH：细胞色素 c 还原酶的维持。在 SD 大鼠肝细胞中，前列腺素对细胞色素 P-450 含量或 NADPH：细胞色素 c 还原酶活性没有影响，而它们阻止了培养后 24 至 72 小时观察到的 MFO 活性增加。
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