Amatruda T T, Bohman R, Ranyard J, Koeffler H P
Blood. 1987 Apr;69(4):1225-36.
We examined the expression of HLA-DR and HLA-DQ antigens and mRNA from myeloid and lymphoid cells obtained from normal volunteers and established cell lines. Cytofluorometric analysis and immunoprecipitation were performed using murine monoclonal antibodies specific for HLA-DR (L-243) and HLA-DQ (Leu 10). The expression of mRNA for HLA-DR and HLA-DQ chains was determined by Northern blot and RNA dot-blot analysis. Lymphoid cell lines expressed both HLA-DR and HLA-DQ antigens, with consistently higher levels of expression of DR. Myeloid cell lines of early myeloblast or bipotent (myeloid-erythroid) phenotype (KG-1, KG-1a, HEL) expressed HLA-DR at high levels, whereas cell lines manifesting a greater degree of myeloid maturation (ML-3, HL-60, U937) expressed DR at low or undetectable levels. The HLA-DQ antigen was expressed at low levels on the surface of KG-1 and KG-1a cells but was not detectable on other myeloid cell lines. The expression of mRNA for HLA-DR and HLA-DQ chains paralleled the pattern of expression of the respective antigens. The HL-60 and U-937 cells stimulated to differentiate in vitro to macrophages with 1,25 dihydroxyvitamin D3 [1,25(OH)2D3] were induced to express detectable levels of HLA-DR antigens. Exposure to gamma-interferon (gamma-IFN) increased the expression of HLA-DR antigens by all myeloid cell lines. Induction of differentiation in vitro with either 1,25(OH)2D3 or dimethyl sulfoxide potentiated this effect of gamma-IFN. Expression of the HLA-DQ antigens was increased on KG-1 myeloblasts after exposure to gamma-IFN. HLA-DQ expression could not be detected on other myeloid cell lines after exposure to gamma-IFN, nor was HLA-DQ expression stimulated by gamma-IFN after HL-60 and U-937 cells were induced to differentiate to macrophagelike cells in vitro. These results provide additional evidence that expression of the HLA-DR and HLA-DQ genes may be independently regulated in human myeloid cells.
我们检测了从正常志愿者和已建立的细胞系中获取的髓系和淋巴系细胞上HLA - DR和HLA - DQ抗原及mRNA的表达情况。使用针对HLA - DR（L - 243）和HLA - DQ（Leu 10）的鼠单克隆抗体进行细胞荧光分析和免疫沉淀。通过Northern印迹和RNA斑点印迹分析确定HLA - DR和HLA - DQ链的mRNA表达。淋巴系细胞系同时表达HLA - DR和HLA - DQ抗原，其中DR的表达水平始终较高。早期成髓细胞或双潜能（髓系 - 红系）表型的髓系细胞系（KG - 1、KG - 1a、HEL）高水平表达HLA - DR，而表现出更高程度髓系成熟的细胞系（ML - 3、HL - 60、U937）则低水平表达或检测不到DR。HLA - DQ抗原在KG - 1和KG - 1a细胞表面低水平表达，但在其他髓系细胞系上检测不到。HLA - DR和HLA - DQ链的mRNA表达与各自抗原的表达模式平行。用1,25 - 二羟维生素D3 [1,25(OH)2D3]体外刺激HL - 60和U - 937细胞分化为巨噬细胞后，诱导其表达可检测水平的HLA - DR抗原。暴露于γ - 干扰素（γ - IFN）可增加所有髓系细胞系中HLA - DR抗原的表达。用1,25(OH)2D3或二甲基亚砜体外诱导分化可增强γ - IFN的这种作用。暴露于γ - IFN后，KG - 1成髓细胞上HLA - DQ抗原的表达增加。暴露于γ - IFN后，在其他髓系细胞系上检测不到HLA - DQ表达，HL - 60和U - 937细胞体外诱导分化为巨噬样细胞后，γ - IFN也未刺激HLA - DQ表达。这些结果提供了额外的证据，表明HLA - DR和HLA - DQ基因的表达在人类髓系细胞中可能受到独立调控。
