State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
J Exp Bot. 2019 Sep 24;70(18):4619-4630. doi: 10.1093/jxb/erz203.
Terpenoids, the biggest class of plant secondary metabolites, have a wide range of significant physiological roles, while many of them are important natural drugs. Biosynthesis of pharmaceutical terpenoids in plants is a fairly complex process, most of which involves cytochrome P450 (CYP450) monooxygenases. CYP450 enzymes are versatile biocatalysts that play critical roles in terpenoid skeleton modification and structural diversity. Therefore, the discovery and identification of CYP450 genes is significant for elucidating the terpenoid biosynthetic pathway. This review summarizes the progress and cloning strategies relating to CYP450s in pharmaceutical terpenoid biosynthesis of the past decade.
萜类化合物是植物次生代谢产物中最大的一类，具有广泛的重要生理作用，其中许多是重要的天然药物。植物中药物萜类化合物的生物合成是一个相当复杂的过程，其中大部分涉及细胞色素 P450（CYP450）单加氧酶。CYP450 酶是多功能的生物催化剂，在萜类骨架修饰和结构多样性方面发挥着关键作用。因此，CYP450 基因的发现和鉴定对于阐明萜类生物合成途径具有重要意义。本综述总结了过去十年中与药物萜类生物合成中 CYP450 相关的研究进展和克隆策略。
