Demonstration Base for International Science & Technology Cooperation of Sichuan Province, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Environ Sci Pollut Res Int. 2019 Jun;26(17):17834-17844. doi: 10.1007/s11356-019-05226-7. Epub 2019 Apr 29.
Selenium (Se) is a vital element which leads to strong antioxidation in animals and humans. However, the mechanism underlying natural cereal Se-induced biological changes is not well understood. This study intended to explore the gene differential expression in naturally aged mice exposed to selenium by RNA-Seq technique. A total spectrum of 53 differentially expressed genes was quantified in mice heart tissues treated with Se-rich and general rice. The GO functional annotation of differentially expressed genes disclosed the enrichment of cellular process, ionic binding, biological regulation, and catalytic activity. One hundred twenty-three differential pathways (cardiovascular diseases, immune system, transport and catabolism, longevity regulating, and PI3K-AKT signaling) were identified according to KEGG metabolic terms. Afterwards, the effect of Se-rich rice on the antioxidant activity was assessed. The selenium-rich diet increased the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in mice serum and livers while significantly reduces methane dicarboxylic aldehyde (MDA) contents. FOXO1 and FOXO3 genes, which acted as the regulators of apoptosis and the antioxidant enzyme, were significantly enhanced in mice when fed with Se-rich rice. In short, the present findings disclosed the alluring insights of organic and inorganic selenium sources on certain biological processes and antioxidant activity of living bodies. However, long-term trials are still required to draw a definitive conclusion, including risks and benefit analysis for various management strategies.
硒（Se）是一种重要的元素，它能导致动物和人类体内的强烈抗氧化作用。然而，自然谷物硒诱导生物变化的机制尚不清楚。本研究旨在通过 RNA-Seq 技术探索自然衰老的小鼠暴露于硒后基因差异表达的情况。在富含硒和普通大米处理的小鼠心脏组织中，共定量了 53 个差异表达基因。差异表达基因的 GO 功能注释揭示了细胞过程、离子结合、生物调节和催化活性的富集。根据 KEGG 代谢术语，鉴定了 123 个差异通路（心血管疾病、免疫系统、运输和分解代谢、长寿调节和 PI3K-AKT 信号通路）。随后，评估了富硒大米对抗氧化活性的影响。富含硒的饮食增加了小鼠血清和肝脏中的总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px），同时显著降低了甲烷二羧酸醛（MDA）的含量。FOXO1 和 FOXO3 基因作为凋亡和抗氧化酶的调节剂，在喂食富硒大米的小鼠中显著增强。总之，本研究结果揭示了有机和无机硒源对生物体某些生物学过程和抗氧化活性的诱人见解。然而，仍需要长期试验来得出明确的结论，包括对各种管理策略的风险和效益分析。
