13B's Research Group, I3Bs-Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal.
2ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Tissue Eng Part B Rev. 2019 Aug;25(4):330-356. doi: 10.1089/ten.TEB.2018.0383.
The main goal of this review is to give an overview of cell-based and tissue engineered strategies for tendon-to-bone interface. The essential role of cells in tendon-to-bone interface development, healing, and regeneration, is underpinned by the physiological status of the junction. Therefore, recent studies underlining the effect of oxygen concentration and production of growth factors are reviewed. A critical view is made on the use of two-dimensional versus three-dimensional culture systems and mechanical stimulation. An overview of advances on bioengineered strategies in light of the biological/cellular requirements of enthesis will contribute to innovations in tendon-to-bone engineering and regeneration.
本综述的主要目的是概述基于细胞和组织工程的策略在肌腱-骨界面中的应用。细胞在肌腱-骨界面的发育、愈合和再生中起着至关重要的作用，这是由连接点的生理状态决定的。因此，本文综述了强调氧浓度和生长因子产生的影响的最新研究。本文还对二维与三维培养系统和机械刺激的应用进行了批判性的讨论。鉴于附着点的生物学/细胞要求，对生物工程策略的进展进行概述将有助于肌腱-骨工程和再生的创新。
