Pereira Rafael, Pereira Anna L, Ferreira Marcilio M, Fontenelle Raquel O S, Saker-Sampaio Silvana, Santos Hélcio S, Bandeira Paulo N, Vasconcelos Mayron A, Queiroz José A N, Braz-Filho Raimundo, Teixeira Edson H
Laboratório Integrado de Biomoléculas/LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Rua Monsenhor Furtado, s/n, Rodolfo Teófilo, 60441-750 Fortaleza, CE, Brazil.
Laboratório de Microbiologia/LABMIC, Departamento de Ciências Biológicas, Universidade Estadual Vale do Acaraú, Av. da Universidade, 850, Campus da Betânia, 62040-370 Sobral, CE, Brazil 3Departamento de Engenharia de Pesca, Centro de Ciências Agrárias, Universidade Federal do Ceará, Av. Mister Hull, 2977, 60356-001 Fortaleza, CE, Brazil.
An Acad Bras Cienc. 2019;91(2):e20180204. doi: 10.1590/0001-3765201920180204. Epub 2019 Apr 25.
This study evaluated the antibacterial, antifungal, and antioxidant effect of 7-hydroxy-4',6-dimethoxy-isoflavone and essential oil of Myroxylon peruiferum. The compound was isolated and its structure elucidated by NMR. The chemical composition of essential oil determined by GC-MS analysis. To evaluation of antimicrobial activity, the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) were performed. In addition to analysis of antioxidant activity, DPPH radical scavenging tests, iron chelating assay (FIC), antioxidant reducing power assay (FRAP) and β-carotene bleaching assay (BCB) were performed. For the essential oil were identified 24 organized compounds having as main constituents; Germacrene D (17.2%), α-pinene (14.8%) and E-caryophyllene (10.8%). The results showed that isoflavone (2000 to 156 μg/mL) and essential oil (5.0 to 1.25%) present antibacterial and antifungal activity against Gram-positive bacteria and filamentous fungi. The isoflavone and the essential oil also presented antioxidant activity in all the tests, mainly on inhibition of the oxidation of β-carotene test concentrations ranging from 60 to 100%. In conclusion, isoflavone and essential oil from M. peruiferum present an antimicrobial alternative against Gram-positive bacteria, especially of the genus Staphylococcus and dermatophyte fungi of the genus Trichophyton, as well as a natural compound antioxidant.
本研究评估了7-羟基-4',6-二甲氧基异黄酮和秘鲁香脂精油的抗菌、抗真菌及抗氧化作用。分离出该化合物并通过核磁共振阐明其结构。通过气相色谱-质谱联用（GC-MS）分析确定了精油的化学成分。为评估抗菌活性，进行了最低抑菌浓度（MIC）、最低杀菌浓度（MBC）和最低杀真菌浓度（MFC）测定。除了分析抗氧化活性外，还进行了二苯基苦味酰基自由基（DPPH）清除试验、铁螯合测定（FIC）、抗氧化还原能力测定（FRAP）和β-胡萝卜素漂白测定（BCB）。对于精油，鉴定出24种有组织的化合物，其主要成分包括：大根香叶烯D（17.2%）、α-蒎烯（14.8%）和反式石竹烯（10.8%）。结果表明，异黄酮（2000至156μg/mL）和精油（5.0至1.25%）对革兰氏阳性菌和丝状真菌具有抗菌和抗真菌活性。异黄酮和精油在所有试验中也都表现出抗氧化活性，主要是在β-胡萝卜素氧化抑制试验中，抑制率范围为60%至100%。总之，秘鲁香脂中的异黄酮和精油是对抗革兰氏阳性菌（尤其是葡萄球菌属）和毛癣菌属皮肤癣菌的抗菌替代品，也是一种天然的化合物抗氧化剂。
