Shay C E, Foster P G, Neelin J M
Comp Biochem Physiol B. 1987;86(1):193-9. doi: 10.1016/0305-0491(87)90197-0.
The relationship between immunological distance (I.D.) measured by microcomplement fixation and amino acid sequence difference for lysine-rich histones was tested using antisera to lysine-rich histones of known sequence, chicken H1 and H5, goose H5, and trout H1 as well as to trout H5. The best relationship between I.D. (y) and percent sequence difference (x) for lysine-rich histones, y = 2x, applies as well to other histones of known sequence but it differs from y = 5x, reported for other proteins and often used for histones. Although deviations indicate that I.D. is a poor predictor of primary sequence differences among histones, it suggests that trout H5 is more closely related to H1 than to chicken H5.
利用针对已知序列的富含赖氨酸组蛋白（鸡的H1和H5、鹅的H5以及鳟鱼的H1）以及鳟鱼H5的抗血清，测试了通过微量补体结合法测得的免疫距离（I.D.）与富含赖氨酸组蛋白氨基酸序列差异之间的关系。富含赖氨酸组蛋白的I.D.（y）与序列差异百分比（x）之间的最佳关系为y = 2x，这同样适用于其他已知序列的组蛋白，但与报道的其他蛋白质（常用于组蛋白）的y = 5x不同。尽管偏差表明I.D.并非组蛋白一级序列差异的良好预测指标，但这表明鳟鱼H5与H1的亲缘关系比与鸡H5的更近。
