聚乙烯亚胺、超声和纳米气泡联合用于增强肝癌中短发夹RNA的递送
Enhanced shRNA delivery by the combination of polyethylenimine, ultrasound, and nanobubbles in liver cancer.
作者信息
Li Hongbo, Wang Ziyu, Zhang Jia, Yuan Chenyan, Zhang Hao, Hou Xinxin, Zhang Dongsheng
机构信息
Department of Ultrasonography, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.
Jiangsu Key Laboratory for Biomaterials and Devices, Medical School, Southeast University, Nanjing, China.
出版信息
Technol Health Care. 2019;27(S1):263-272. doi: 10.3233/THC-199025.
BACKGROUND
Traditional cancer treatments such as surgery, radiation, and chemotherapy destroy both cancer and normal cells, which limit their clinical application. It is difficult to achieve the best results for any liver cancer patients using any single treatment method. Gene therapy for HCC demands non-invasive, efficient, targeted and safe gene transfection strategies.
OBJECTIVE
In this study, a nonviral shRNA gene delivery system utilizing a combination of PEI, US, and NBs was developed for targeting survivin in liver Cancer.
METHODS AND RESULTS
The PEI-shRNA-NBs cumulated in the tumor tissue because of the EPR effect. By exposure to the US, micelles shRNA may be released from PEI-shRNA-NBs in tumor tissues and the shRNA then transmitted efficiently to cancer cells. Considerably enhanced therapeutic outcome was obtained with the gene silencing effect enhanced.
CONCLUSIONS
PEI-shRNA-NBs possess the potential to become promising tools intended for shRNA delivery.
背景
传统的癌症治疗方法，如手术、放疗和化疗，会同时破坏癌细胞和正常细胞，这限制了它们的临床应用。使用任何单一治疗方法都很难为所有肝癌患者取得最佳效果。肝癌的基因治疗需要非侵入性、高效、靶向和安全的基因转染策略。
目的
在本研究中，开发了一种利用聚乙烯亚胺（PEI）、超声（US）和纳米气泡（NBs）组合的非病毒短发夹RNA（shRNA）基因递送系统，用于靶向肝癌中的生存素。
方法与结果
由于增强渗透滞留（EPR）效应，PEI-shRNA-NBs在肿瘤组织中蓄积。通过超声照射，纳米粒中的shRNA可能在肿瘤组织中从PEI-shRNA-NBs释放出来，然后shRNA有效地传递到癌细胞中。基因沉默效果增强，治疗效果显著提高。
结论
PEI-shRNA-NBs有潜力成为用于递送shRNA的有前景的工具。
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