Department of Inorganic Materials, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.
Hunan Key Lab of Mineral Materials and Application, Central South University, Changsha, 410083, China.
Sci Rep. 2019 May 2;9(1):6808. doi: 10.1038/s41598-019-41093-5.
DNA purification is essential for the detection of human clinical specimens. A non-destructive, controllable, and low reagent consuming DNA extraction method is described. Negatively charged DNA is absorbed onto a negatively charged montmorillonite to achieve non-destructive DNA extraction based on cation bridge construction and electric double layer formation. Different valence cation modified montmorillonite forms were used to validate the charge-dependent nature of DNA adsorption on montmorillonite. Electric double layer thickness thinning/thickening with the high/lower valence cations exists, and the minerals tended to be sedimentation-stable due to the Van der Waals attraction/electrostatic repulsion. Li-modified montmorillonite with the lowest charge states showed the best DNA adsorption efficiency of 8-10 ng/μg. Charge-dependent regulating research provides a new perspective for controllable DNA extraction and a deep analysis of interface engineering mechanisms.
DNA 纯化对于人类临床标本的检测至关重要。本文描述了一种非破坏性、可控制且试剂消耗低的 DNA 提取方法。带负电荷的 DNA 被吸附到带负电荷的蒙脱石上，基于阳离子桥的构建和双电层的形成，实现了非破坏性的 DNA 提取。使用不同价态阳离子修饰的蒙脱石形成物来验证 DNA 在蒙脱石上吸附的电荷依赖性。存在着双电层厚度的变薄/变厚与高/低价态阳离子，由于范德华吸引力/静电排斥，矿物趋于沉降稳定。电荷状态最低的 Li 修饰蒙脱石表现出最佳的 DNA 吸附效率，可达 8-10ng/μg。这种电荷依赖性调控研究为可控 DNA 提取提供了新视角，并深入分析了界面工程机制。
