一种含有分层硅酸钠片层的简单热塑性基底，用于高分子量 DNA 提取。
A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction.
机构信息
Biomedical Engineering Department, Johns Hopkins University, Baltimore, MD 21218, USA.
Institute of Bioengineering and Nanotechnology, Agency of Science Technology and Research, Singapore, 138669, Singapore.
出版信息
Adv Mater. 2016 Dec;28(48):10630-10636. doi: 10.1002/adma.201603738. Epub 2016 Nov 8.
Abstract
An inexpensive, magnetic thermoplastic nanomaterial is developed utilizing a hierarchical layering of micro- and nanoscale silica lamellae to create a high-surface-area and low-shear substrate capable of capturing vast amounts of ultrahigh-molecular-weight DNA. Extraction is performed via a simple 45 min process and is capable of achieving binding capacities up to 1 000 000 times greater than silica microparticles.
摘要
开发了一种廉价的磁性热塑性纳米材料，利用微纳尺度二氧化硅片的分层结构，形成具有高比表面积和低剪切力的基底，能够捕获大量超高分子量的 DNA。提取过程简单，只需 45 分钟，并且比硅胶微球的结合容量高 100 万倍。
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