大肠杆菌L11核糖体蛋白操纵子的翻译调控：确定L1抑制作用靶位点的突变
Translational regulation of the L11 ribosomal protein operon of Escherichia coli: mutations that define the target site for repression by L1.
作者信息
Thomas M S, Nomura M
出版信息
Nucleic Acids Res. 1987 Apr 10;15(7):3085-96. doi: 10.1093/nar/15.7.3085.
Abstract
The L11 ribosomal protein operon of Escherichia coli contains the genes for L11 and L1 and is feedback regulated by the translational repressor L1. The mRNA target site for this repression is located close to the Shine-Dalgarno sequence for the first cistron, rp1K (L11). By use of a random mutagenesis procedure we have isolated and characterized a series of point mutations in the L11 leader mRNA which eliminate or greatly diminish the regulation by L1. The mutations define a region essential for translational regulation upstream of the L11 Shine-Dalgarno sequence and identify a region of structural homology with the L1 binding site on 23S rRNA. These results are also consistent with the previously proposed model for the secondary structure of the L11 leader mRNA.
摘要
大肠杆菌的L11核糖体蛋白操纵子包含L11和L1的基因，并受翻译阻遏物L1的反馈调节。这种阻遏作用的mRNA靶位点位于第一个顺反子rp1K（L11）的Shine-Dalgarno序列附近。通过随机诱变程序，我们分离并鉴定了L11前导mRNA中的一系列点突变，这些突变消除或大大减弱了L1的调节作用。这些突变定义了L11 Shine-Dalgarno序列上游翻译调节所必需的区域，并确定了与23S rRNA上L1结合位点的结构同源区域。这些结果也与先前提出的L11前导mRNA二级结构模型一致。
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1
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Proc Natl Acad Sci U S A. 1981 Jan;78(1):167-71. doi: 10.1073/pnas.78.1.167.
2
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Cell. 1981 Apr;24(1):243-9. doi: 10.1016/0092-8674(81)90520-1.
3
Regulation of synthesis of Escherichia coli ribosomal proteins L1 and L11.大肠杆菌核糖体蛋白L1和L11合成的调控
Arch Biochem Biophys. 1981 Jan;206(1):51-3. doi: 10.1016/0003-9861(81)90064-3.
4
The secondary structure of the protein L1 binding region of ribosomal 23S RNA. Homologies with putative secondary structures of the L11 mRNA and of a region of mitochondrial 16S rRNA.核糖体23S RNA的蛋白质L1结合区域的二级结构。与L11 mRNA的假定二级结构以及线粒体16S rRNA一个区域的同源性。
Nucleic Acids Res. 1981 Jan 24;9(2):293-307. doi: 10.1093/nar/9.2.293.
5
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.通过在大肠杆菌中进行DNA融合和克隆分析基因控制信号。
J Mol Biol. 1980 Apr;138(2):179-207. doi: 10.1016/0022-2836(80)90283-1.
6
In vitro expression of Escherichia coli ribosomal protein genes: autogenous inhibition of translation.大肠杆菌核糖体蛋白基因的体外表达：翻译的自体抑制
Proc Natl Acad Sci U S A. 1980 Apr;77(4):1837-41. doi: 10.1073/pnas.77.4.1837.
7
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8
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9
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