Baughman G, Nomura M
Cell. 1983 Oct;34(3):979-88. doi: 10.1016/0092-8674(83)90555-x.
The L11 ribosomal protein operon in Escherichia coli consists of the structural genes for proteins L11 and L1. Hybrid deletion plasmids were constructed carrying these two genes with decreasing amounts of the leader mRNA under lac transcriptional control. Measurements of mRNA and protein synthesis directed by these plasmids in vitro and in vivo demonstrate that the regulation of this operon is posttranscriptional and identifies a region of the mRNA, preceding the proximal L11 gene, important for successful feedback inhibition of L11 and L1 synthesis by L1. Additionally, deletions extending to the ribosome binding site of the L11 gene fail to synthesize both L11 and the downstream L1 protein although synthesis of the corresponding mRNA remains unchanged. These results directly demonstrate the presence of translational coupling in this bicistronic operon.
大肠杆菌中的L11核糖体蛋白操纵子由蛋白质L11和L1的结构基因组成。构建了携带这两个基因的杂种缺失质粒，这些质粒在乳糖转录控制下，所含前导mRNA的量逐渐减少。对这些质粒在体外和体内指导的mRNA和蛋白质合成的测量表明，该操纵子的调控是转录后调控，并确定了mRNA上位于近端L11基因之前的一个区域，该区域对于L1对L11和L1合成的成功反馈抑制很重要。此外，延伸至L11基因核糖体结合位点的缺失不能合成L11和下游的L1蛋白，尽管相应mRNA的合成保持不变。这些结果直接证明了这个双顺反子操纵子中存在翻译偶联。
