Smit Pieter W, Heijman Titia, Abdallaoui Meriem El, Bruisten Sylvia M
Department of Infectious Diseases, Public Health Service Amsterdam, Amsterdam, the Netherlands.
Amsterdam UMC, University of Amsterdam, Medical Microbiology, Meibergdreef 9, Amsterdam, the Netherlands.
Heliyon. 2019 Apr 24;5(4):e01522. doi: 10.1016/j.heliyon.2019.e01522. eCollection 2019 Apr.
The detection of herpes simplex viruses and from genital lesions requires efficient sampling of genetic material for a reliable molecular diagnosis. From 460 patients attending the Public Health clinic, two swabs (dry cotton swabs and Eswabs) per patient were collected in alternating order from the same lesion. Additionally, three storage conditions of Eswabs up to 28 days were evaluated to assess the stability of DNA over time. Out of the 830 PCRs performed, 20 (2.4%) PCRs were discordant between the two swabs. No significant differences were observed between the two sample types. HSV1 and HSV2 could be reliably detected from Eswabs up to 28 days when kept at room temperature. A single swab from a genital lesion is sufficient for reliable diagnosis of α-herpes viruses and , for which both a dry cotton swab or Eswab could be used.
从生殖器病变中检测单纯疱疹病毒需要高效采集遗传物质，以进行可靠的分子诊断。在460名到公共卫生诊所就诊的患者中，从同一病变处交替采集每名患者的两支拭子（干棉拭子和Eswab拭子）。此外，评估了Eswab拭子长达28天的三种储存条件，以评估DNA随时间的稳定性。在进行的830次聚合酶链反应（PCR）中，两支拭子之间有20次（2.4%）PCR结果不一致。两种样本类型之间未观察到显著差异。当在室温下保存时，长达28天的Eswab拭子中均可可靠检测到HSV1和HSV2。来自生殖器病变的单个拭子足以对α疱疹病毒进行可靠诊断，干棉拭子或Eswab拭子均可用于此诊断。
