Heylings J R, Feldman M
Prostaglandins. 1986 Dec;32(6):907-17. doi: 10.1016/0090-6980(86)90098-5.
This study investigated the action of enprostil, a synthetic analog of PGE2, on gastric HCO3- secretion in humans and on duodenal HCO3- secretion in the anesthetized rat. A previously validated 2-component model was used to calculate gastric HCO3- and H+ secretion in 10 human subjects. Compared to placebo, a single 70 micrograms oral dose of enprostil increased basal gastric HCO3- secretion from 1810 +/- 340 to 3190 +/- 890 mumol/hr (P less than 0.05). In addition, enprostil reduced basal gastric H+ secretion from 5240 +/- 1140 to 1680 +/- 530 mumol/hr (P less than 0.02). Enprostil also increased HCO3- secretion and reduced H+ secretion during intravenous pentagastrin infusion. In the rat, duodenal HCO3- secretion was measured by direct titration in situ using perfused segments of duodenum just distal to the Brunner gland area and devoid of pancreatic and biliary secretions. Addition of enprostil (10 micrograms/ml) to the duodenal bathing solution increased duodenal HCO3- secretion from 6.3 +/- 1.3 to 15.1 +/- 2.0 mumol/cm X hr (P less than 0.01, n = 6). The stimulatory action of enprostil on duodenal HCO3- secretion at 10 micrograms/ml was comparable in magnitude and duration to that of 10 micrograms/ml natural PGE2. In summary, the PGE2 analog enprostil stimulated gastroduodenal HCO3- secretion, effects which may be beneficial in protection of the gastroduodenal mucosa against luminal acid.
本研究调查了前列腺素E2的合成类似物恩前列素对人体胃HCO3-分泌以及对麻醉大鼠十二指肠HCO3-分泌的作用。采用先前验证的双组分模型计算10名人类受试者的胃HCO3-和H+分泌。与安慰剂相比，单次口服70微克恩前列素可使基础胃HCO3-分泌从1810±340微摩尔/小时增加至3190±890微摩尔/小时（P<0.05）。此外，恩前列素可使基础胃H+分泌从5240±1140微摩尔/小时降至1680±530微摩尔/小时（P<0.02）。在静脉注射五肽胃泌素期间，恩前列素也增加了HCO3-分泌并减少了H+分泌。在大鼠中，通过直接原位滴定法测量十二指肠HCO3-分泌，使用位于布伦纳腺区域远端且无胰液和胆汁分泌的十二指肠灌注段。向十二指肠灌洗液中添加恩前列素（10微克/毫升）可使十二指肠HCO3-分泌从6.3±1.3微摩尔/厘米×小时增加至15.1±2.0微摩尔/厘米×小时（P<0.01，n=6）。10微克/毫升恩前列素对十二指肠HCO3-分泌的刺激作用在强度和持续时间上与10微克/毫升天然前列腺素E2相当。总之，前列腺素E2类似物恩前列素刺激胃十二指肠HCO3-分泌，这些作用可能有助于保护胃十二指肠黏膜免受腔内酸的损伤。
