Shorrock C J, Gibbons L C, Rees W D
Department of Gastroenterology, Hope Hospital, University of Manchester School of Medicine, Salford, U.K.
Dig Dis Sci. 1989 Jul;34(7):1016-20. doi: 10.1007/BF01536366.
The protective and ulcer healing properties of prostaglandins are well established. We have explored the possible mode of action of enprostil, a synthetic dehydroprostaglandin E2, on amphibian gastroduodenal mucosal bicarbonate secretion in vitro and on human gastric bicarbonate secretion in vivo. Addition of enprostil (10(-6) M) to the luminal solution of isolated amphibian gastric mucosa produced a 28% increase in bicarbonate secretion without a change in transmucosal potential difference. The same concentration of enprostil added to the luminal solution of isolated amphibian duodenal mucosa produced a 37% increase in bicarbonate secretion and was associated with a rise in transmucosal potential difference. The gastric output of bicarbonate from the human stomach has been calculated using a perfusion technique before, during, and after perfusion with enprostil (35 micrograms) in six healthy volunteers. A significant 78% increase in bicarbonate secretion occurred during the period of enprostil perfusion, falling to normal during the postenprostil period. These changes were caused mainly by an increase in gastric secretory volume with insignificant increases in bicarbonate concentration. These results suggest that stimulation of gastroduodenal bicarbonate secretion by enprostil may play a role in its protective actions.
前列腺素的保护和促进溃疡愈合特性已得到充分证实。我们探讨了合成的脱氢前列腺素E2（恩前列素）对两栖类动物胃十二指肠黏膜碳酸氢盐分泌的体外作用方式以及对人体胃碳酸氢盐分泌的体内作用方式。向离体两栖类动物胃黏膜的腔内溶液中添加恩前列素（10⁻⁶ M），可使碳酸氢盐分泌增加28%，而跨黏膜电位差无变化。向离体两栖类动物十二指肠黏膜的腔内溶液中添加相同浓度的恩前列素，可使碳酸氢盐分泌增加37%，并伴有跨黏膜电位差升高。采用灌注技术计算了6名健康志愿者在灌注恩前列素（35微克）之前、期间和之后人胃中碳酸氢盐的输出量。在恩前列素灌注期间，碳酸氢盐分泌显著增加78%，在灌注后阶段降至正常水平。这些变化主要是由于胃分泌量增加所致，而碳酸氢盐浓度增加不明显。这些结果表明，恩前列素刺激胃十二指肠碳酸氢盐分泌可能在其保护作用中发挥作用。
