Shen Dan, Wei Jianzi, Chen Lianjing, Shen Xueyong, Wang Lina
1 School of Acupuncture, Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
2 Shanghai Research Center for Acupuncture and Meridians, Shanghai, China.
Photobiomodul Photomed Laser Surg. 2019 Mar;37(3):151-158. doi: 10.1089/photob.2018.4570.
Improvement of microcirculation is one of the important mechanisms of low-level laser therapy (LLLT) to treat some diseases such as wound healing. Most previous studies have been carried out with multiple lasers other than the 10,600-nm CO laser. Recently, the CO laser has been used not only as a tool for excision of soft tissues but also for therapeutic applications. To study whether low-level CO laser irradiation can influence microcirculation and further explore the underlying mechanisms. Seventy-milliwatt (70-mW) CO lasers irradiated the forearms of 12 participants and skin blood perfusion (SkBP) was measured with a laser speckle imager. The thermal effect of irradiation was evaluated by measuring the irradiated skin in vivo and the exposed cell suspensions in vitro. Extracellular adenosine triphosphate (eATP) of the human mast cell line (HMC-1) is assessed by luciferin-luciferase assay to explore the potential mechanisms. Irradiation caused dose-dependent increase in SkBP. At a medium dose of 262 J/cm, SkBP reached its maximum value at 195.8% ± 18.6% of the baseline ( = 12, < 0.01). Such laser irradiation had a mild thermal effect, heating local skin temperature (SkT) by 6.1°C ± 0.3°C ( = 10) and warming cell suspensions by 4.5°C ± 0.8°C ( = 6). Irradiation dose-dependently lowered eATP levels of HMC-1 cells in vitro. At a medium dose of 262 J/cm, eATP levels declined to the minimum at 74.8% ± 5.5% of the baseline ( = 12, < 0.01). This downregulation effect could be significantly inhibited by 100-μM ARL67156, a nonspecific ecto-ATPase inhibitor. On the contrary, heating itself slightly raised the level of eATP. Low-level CO laser irradiation can improve microcirculation. Besides the thermal effect, regulation of extravascular eATP by the photobiomodulation mechanism may be involved. This implies that CO lasers might be used in LLLT.
改善微循环是低强度激光疗法（LLLT）治疗诸如伤口愈合等一些疾病的重要机制之一。此前大多数研究使用的是除10600纳米CO激光之外的多种激光。最近，CO激光不仅被用作软组织切除工具，还用于治疗应用。为了研究低强度CO激光照射是否会影响微循环并进一步探究其潜在机制，用70毫瓦（70 - mW）的CO激光照射12名参与者的前臂，并用激光散斑成像仪测量皮肤血流灌注（SkBP）。通过在体内测量照射的皮肤以及在体外测量暴露的细胞悬液来评估照射的热效应。通过荧光素 - 荧光素酶测定法评估人肥大细胞系（HMC - 1）的细胞外三磷酸腺苷（eATP），以探究潜在机制。照射导致SkBP呈剂量依赖性增加。在262 J/cm的中等剂量下，SkBP达到其最大值，为基线的195.8%±18.6%（n = 12，P < 0.01）。这种激光照射具有轻微的热效应，使局部皮肤温度（SkT）升高6.1°C±0.3°C（n = 10），并使细胞悬液温度升高4.5°C±0.8°C（n = 6）。照射在体外使HMC - 1细胞的eATP水平呈剂量依赖性降低。在262 J/cm的中等剂量下，eATP水平降至最低，为基线的74.8%±5.5%（n = 12，P < 0.01）。这种下调作用可被100 μM ARL67156（一种非特异性胞外ATP酶抑制剂）显著抑制。相反，加热本身会使eATP水平略有升高。低强度CO激光照射可改善微循环。除了热效应外，光生物调节机制对血管外eATP的调节可能也参与其中。这意味着CO激光可能用于低强度激光疗法。
