Bioengineering Research Group, Nanotechnology & Advanced Materials Department, Materials & Energy Research Center (MERC), Tehran, Iran.
Bioengineering Research Group, Nanotechnology & Advanced Materials Department, Materials & Energy Research Center (MERC), Tehran, Iran; Cellular and Molecular Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran; Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran.
Methods. 2020 Jan 15;171:108-118. doi: 10.1016/j.ymeth.2019.04.019. Epub 2019 Apr 30.
Decellularization aims to remove cells from tissue ultrastructure while preserving the mechanical and biological properties, which makes the decellularized extracellular matrix (dECM) an appropriate scaffold for tissue engineering applications. Three-dimensional (3D) bioprinting technology as a reproducible and accurate method can print the combination of ECM and autologous cells layer by layer to fabricate patient based cell-laden structures representing the intrinsic cues of natural ECM. This review defines ECM, classifies decellularization agents and techniques, and explains different sources of ECM. Then, bioprinting techniques, bioink concept, applications of dECM bioinks, and finally the future perspectives of 3d bioprinting technology are discussed.
脱细胞化旨在去除组织超微结构中的细胞，同时保留机械和生物学特性，这使得脱细胞细胞外基质 (dECM) 成为组织工程应用的合适支架。三维 (3D) 生物打印技术作为一种可重复和精确的方法，可以逐层打印 ECM 和自体细胞的组合，以制造基于患者的细胞负载结构，代表天然 ECM 的内在线索。本综述定义了细胞外基质，对脱细胞化试剂和技术进行了分类，并解释了细胞外基质的不同来源。然后，讨论了生物打印技术、生物墨水概念、dECM 生物墨水的应用，以及 3D 生物打印技术的未来展望。
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