Yan Jianing, Xu Yi
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Nov 25;37(11):4024-4035. doi: 10.13345/j.cjb.210091.
Decellularized extracellular matrix (dECM), which contains many proteins and growth factors, can provide three-dimensional scaffolds for cells and regulate cell regeneration. 3D bioprinting can print the combination of dECM and autologous cells layer by layer to construct the tissue structure of carrier cells. In this paper, the preparation methods of tissue and organ dECM bioink from different sources, including decellularization, crosslinking, and the application of dECM bioink in bioprinting are reviewed, with future applications prospected.
去细胞细胞外基质（dECM）含有多种蛋白质和生长因子，可为细胞提供三维支架并调节细胞再生。3D生物打印可以逐层打印dECM与自体细胞的组合，以构建载体细胞的组织结构。本文综述了不同来源的组织和器官dECM生物墨水的制备方法，包括去细胞化、交联，以及dECM生物墨水在生物打印中的应用，并对其未来应用进行了展望。
