Bahlol Hagar S, Foda Mohamed F, Ma Jing, Han Heyou
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, China.
Department of Biochemistry, Faculty of Agriculture, Benha University, Moshtohor, Toukh 13736, Egypt.
Nanomaterials (Basel). 2019 May 1;9(5):674. doi: 10.3390/nano9050674.
Triangular silver nanocrystals, well-known as nanoprisms (Ag-NPrs), were successfully developed via a robust and straightforward direct chemical reduction synthetic approach, producing desirable tiny and well-controlled Ag-NPrs. This procedure was accomplished by fabricating a mixture of di-sodium succinate hexa-hydrate (DSSH) and tri-sodium citrate di-hydrate (TSCD) as capping agents at optimal synthetic conditions and under an open-air condition, which proved to be an enormous challenge. Additionally, the Ag-NPrs were fully characterized by UV-vis spectra, X-ray diffraction (XRD), scanning electron microscope (SEM), and dynamic light scattering (DLS). Likewise, the formation stages from spherical silver nanoparticles (Ag-NPs) to triangular Ag-NPrs were also captured simultaneously via transmission electron microscope (TEM) and high-resolution transmission electron microscope (HR-TEM) images. More interestingly, an active thin silica-shell was efficiently applied on the Ag-NPrs outer-layer to increase their functionality. Furthermore, to confirm their biocompatibility, we also carried out cell viability assays for the Ag-NPs, Ag-NPrs, and Ag-NPrs@SiO with different concentrations at 62.5, 125, and 250 µg/mL after 12, 24, and 48 h of exposure time, respectively, on a regular African green monkey kidney cell line. The cell viability test results exemplified that the three silver nanostructures were toxic-free and suitable for further potential biological applications in the near future.
三角形银纳米晶体，即众所周知的纳米棱镜（Ag-NPrs），通过一种稳健且直接的直接化学还原合成方法成功制备出来，得到了理想的微小且可控的Ag-NPrs。该过程是在最佳合成条件下于露天环境中，通过制备六水合丁二酸钠（DSSH）和二水合柠檬酸三钠（TSCD）的混合物作为封端剂来完成的，这被证明是一项巨大的挑战。此外，通过紫外可见光谱、X射线衍射（XRD）、扫描电子显微镜（SEM）和动态光散射（DLS）对Ag-NPrs进行了全面表征。同样，通过透射电子显微镜（TEM）和高分辨率透射电子显微镜（HR-TEM）图像同时捕捉到了从球形银纳米颗粒（Ag-NPs）到三角形Ag-NPrs的形成阶段。更有趣的是，在Ag-NPrs外层高效地施加了一层活性二氧化硅壳以增加其功能。此外，为了确认它们的生物相容性，我们还分别在正常的非洲绿猴肾细胞系上，在暴露时间为12、24和48小时后，对不同浓度（62.5、125和250 µg/mL）的Ag-NPs、Ag-NPrs和Ag-NPrs@SiO进行了细胞活力测定。细胞活力测试结果表明，这三种银纳米结构无毒，适合在不久的将来进一步用于潜在的生物应用。
