Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, 14260, USA.
Department of Chemical and Biological Engineering and Clinical & Translational Research Center, State University of New York at Buffalo, Buffalo, NY, 14260, USA.
Nat Commun. 2019 May 3;10(1):2051. doi: 10.1038/s41467-019-10067-6.
Blood clotting at the vascular injury site is a complex process that involves platelet adhesion and clot stiffening/contraction in the milieu of fluid flow. An integrated understanding of the hemodynamics and tissue mechanics regulating this process is currently lacking due to the absence of an experimental system that can simultaneously model clot formation and measure clot mechanics under shear flow. Here we develop a microfluidic-integrated microclot-array-elastometry system (clotMAT) that recapitulates dynamic changes in clot mechanics under physiological shear. Treatments with procoagulants and platelet antagonists and studies with diseased patient plasma demonstrate the ability of the system to assay clot biomechanics associated with common antiplatelet treatments and bleeding disorders. The changes of clot mechanics under biochemical treatments and shear flow demonstrate independent yet equally strong effects of these two stimulants on clot stiffening. This microtissue force sensing system may have future research and diagnostic potential for various bleeding disorders.
血管损伤部位的血栓形成是一个复杂的过程，涉及血小板黏附和在血流环境中的血栓变硬/收缩。由于缺乏能够在剪切流下同时模拟血栓形成和测量血栓力学的实验系统，因此目前缺乏对调节该过程的血液动力学和组织力学的综合理解。在这里，我们开发了一种微流控集成微血栓阵列弹性测量系统（clotMAT），该系统可以在生理剪切下再现血栓力学的动态变化。用促凝剂和血小板拮抗剂进行处理，并使用患病患者的血浆进行研究，表明该系统能够检测与常见抗血小板治疗和出血性疾病相关的血栓生物力学。在生化处理和剪切流下，血栓力学的变化表明这两种刺激物对血栓变硬具有独立且同样强大的影响。这种微组织力传感系统可能具有未来针对各种出血性疾病的研究和诊断潜力。
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