School of Ophthalmology & Optometry, Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China.
Wenzhou Institute of Biomaterials and Engineering, CAS, Wenzhou, Zhejiang, 325011, China.
Sci Rep. 2019 May 3;9(1):6863. doi: 10.1038/s41598-019-42186-x.
Gelatin methacryloyl (GelMA) is a versatile material for a wide range of bioapplications. There is an intense interest in developing effective chemical strategies to prepare GelMA with a high degree of batch-to-batch consistency and controllability in terms of methacryloyl functionalization and physiochemical properties. Herein, we systematically investigated the batch-to-batch reproducibility and controllability of producing GelMA (target highly and lowly substituted versions) via a one-pot strategy. To assess the GelMA product, several parameters were evaluated, including the degree of methacryloylation, secondary structure, and enzymatic degradation, along with the mechanical properties and cell viability of GelMA hydrogels. The results showed that two types of target GelMA with five batches exhibited a high degree of controllability and reproducibility in compositional, structural, and functional properties owing to the highly controllable one-pot strategy.
明胶甲基丙烯酰（GelMA）是一种多功能材料，可广泛应用于生物领域。人们强烈希望开发有效的化学策略，以制备具有高批次一致性和在甲基丙烯酰官能化和物理化学性质方面可控性的 GelMA。在此，我们通过一锅法策略系统地研究了生产 GelMA（目标高度和低度取代版本）的批次间重现性和可控性。为了评估 GelMA 产品，评估了包括甲基丙烯酰化程度、二级结构和酶降解在内的多个参数，以及 GelMA 水凝胶的机械性能和细胞活力。结果表明，两种类型的目标 GelMA 具有五个批次，由于采用了高度可控的一锅法策略，其在组成、结构和功能性质方面具有高度的可控性和重现性。
