Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Private Bag X3, Johannesburg, 2050, South Africa.
Department of Chemistry, Centre for Analysis and Synthesis, Lund University, P.O. Box 124, 22100, Lund, Sweden.
Anal Bioanal Chem. 2019 Jun;411(16):3685-3693. doi: 10.1007/s00216-019-01850-x. Epub 2019 May 4.
An ultrahigh-pressure supercritical fluid extraction method was optimized and applied to extract seed oil lipids from two moringa species, namely Moringa oleifera (MO) and Moringa peregrina (MP). A full-factorial design was used to investigate the direct and interaction influence of pressure and temperature in the range of 40 to 80 MPa and 40 to 70 °C, respectively, on the extracted amount of oil from crushed seeds. The results revealed that pressure has a significant positive influence on the extracted amount of oil. The best extraction condition using neat CO was found at 80 MPa and 57 °C, yielding 396 ± 23 and 529 ± 26 mg oil per gram of seeds for MO and MP, respectively. An extraction kinetics study revealed a mainly solubility-controlled extraction of oil, and 28 g of CO was required to extract 400 mg of oil per gram of seeds of MO using the developed method. Addition of ethanol to the sample prior to the extraction increased the proportion of extractable polar lipids as well as the total amount of extracted oil. The developed method increased the extracted amount of oil twofold compared to a reference method based on solvent sonication. The obtained oil consisted mainly of glycerolipids, sterol esters, and phospholipids. Phospholipids, campesterol, and stigmasterol ester concentrations were found to be higher in MO while cholesterol ester was more abundant in MP.
采用全因子设计考察了压力（40-80 MPa）和温度（40-70°C）在超临界条件下对两种辣木种子油提取量的直接和交互影响，优化了超高压超临界流体萃取法，并将其应用于从两种辣木（辣木 Moringa oleifera (MO) 和辣木 Moringa peregrina (MP)）的种子中提取油。结果表明，压力对油的提取量有显著的积极影响。在纯 CO2 条件下，最佳提取条件为 80 MPa 和 57°C，MO 和 MP 的种子出油量分别为 396±23 和 529±26 mg/g。萃取动力学研究表明，油的萃取主要受溶解度控制，使用开发的方法从每克 MO 种子中提取 400mg 油需要 28g CO2。在萃取前向样品中添加乙醇可增加可提取极性脂质的比例和总出油量。与基于溶剂超声的参考方法相比，该方法使油的提取量增加了一倍。所得油主要由甘油酯、甾醇酯和磷脂组成。MO 中的磷脂、菜油甾醇和豆甾醇酯浓度较高，而 MP 中的胆固醇酯含量较高。
