用于副溶血性弧菌这种海洋细菌的穿梭克隆载体。
Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus.
作者信息
Datta A R, Kaper J B, MacQuillan A M
出版信息
J Bacteriol. 1984 Nov;160(2):808-11. doi: 10.1128/jb.160.2.808-811.1984.
Abstract
Two cosmid cloning vectors containing lambda cos sequences and a 42-base-pair multipurpose cloning sequence were constructed. pAD22 also contains a 1.4-kilobase TRP-ARS fragment from Saccharomyces cerevisiae. These cosmids transformed Escherichia coli and S. cerevisiae cells and could be mobilized into Vibrio parahaemolyticus strains with a conjugative plasmid, pRK2013. The cosmid pAD22 was genetically and structurally stable during passage through V. parahaemolyticus and E. coli strains.
摘要
构建了两个含有λ cos序列和一个42个碱基对的多用途克隆序列的黏粒克隆载体。pAD22还包含来自酿酒酵母的一个1.4千碱基的TRP-ARS片段。这些黏粒转化了大肠杆菌和酿酒酵母细胞，并且可以通过接合质粒pRK2013转入副溶血性弧菌菌株。黏粒pAD22在通过副溶血性弧菌和大肠杆菌菌株传代过程中在遗传和结构上是稳定的。
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