Institute of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Institute of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Food Chem. 2019 Sep 15;292:98-105. doi: 10.1016/j.foodchem.2019.04.050. Epub 2019 Apr 12.
Accurate and early diagnosis of mycotoxin is particularly significant to the food and agricultural product safety. In the present work, a sensitive and effective monitoring method for zearalenone (ZEN) was exploited based on a novel self-enhanced electrochemiluminescence (ECL) aptasensor. The self-enhanced lumonophore was compounded by electrostatically combining amine-functionalized Ru(bpy)-doped silica nanoparticles (NH-Ru@SiO NPs) and nitrogen doped graphene quantum dots (NGQDs) together. Since the emitter and co-reactant simultaneously existed in the same nanoparticle, shortened electron-transfer distance and decreased energy loss was obtained. Therefore, self-enhanced ECL aptasensor based on the novel complex expressed the widest linear range of 10 fg mL-10 ng mL and the lowest detection limit of 1 fg mL for ZEN detection. More importantly, ZEN produced during the mildew process of corn flour was monitored by the developed aptasensor, which exhibited superior determination and potential application in real samples.
准确和早期的霉菌毒素诊断对食品安全和农产品安全尤为重要。在本工作中，基于一种新型的自增强电化学发光（ECL）适体传感器，开发了一种用于玉米赤霉烯酮（ZEN）的灵敏有效的监测方法。自增强发光体通过静电结合胺功能化的 Ru（bpy）掺杂硅纳米粒子（NH-Ru@SiO2 NPs）和氮掺杂石墨烯量子点（NGQDs）复合而成。由于发射器和共反应物同时存在于同一纳米粒子中，因此可以缩短电子转移距离并减少能量损失。因此，基于新型复合物的自增强 ECL 适体传感器对 ZEN 的检测表现出最宽的线性范围为 10 fg/mL-10 ng/mL，最低检测限为 1 fg/mL。更重要的是，通过开发的适体传感器监测了玉米面霉变过程中产生的 ZEN，该传感器在实际样品中表现出了优越的测定能力和潜在的应用价值。
