Doctoral Degree Program in Marine Biotechnology, The College of Life Sciences, National Taiwan Ocean University, Keelung, Taiwan; Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei, Taiwan.
Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
Fish Shellfish Immunol. 2019 Jul;90:141-149. doi: 10.1016/j.fsi.2019.03.063. Epub 2019 May 2.
Metamorphosis is a transformation process in larval development associated with changes in morphological and physiological features, including the immune system. The gastrointestinal tract harbors a plethora of bacteria, which might affect the digestion and absorption of nutrients, immunity, and gut-brain crosstalk in the host. In this study, we have performed metagenomic and transcriptomic analyses on the intestines of grouper at the pre-, mid- and post-metamorphosis stages. The sequencing data of 16S rRNA gene showed drastic changes in the microbial communities at different developmental stages. The transcriptomic data revealed that the leukocyte transendothelial migration and the phagosome pathways might play important roles in mediating immunity in grouper at the three developmental stages. This information will increase our understanding of the metamorphosis process in grouper larvae, and shed light on the development of antimicrobial strategy during larval development.
变态发育是幼虫发育过程中的一种转化过程，与形态和生理特征的变化有关，包括免疫系统。肠道中栖息着大量的细菌，这些细菌可能会影响宿主的消化和吸收营养、免疫和肠脑交流。在这项研究中，我们对处于变态发育前、中、后阶段的石斑鱼肠道进行了宏基因组和转录组分析。16S rRNA 基因的测序数据显示，不同发育阶段的微生物群落发生了剧烈变化。转录组数据表明，白细胞跨内皮迁移和吞噬体途径可能在介导石斑鱼三个发育阶段的免疫中发挥重要作用。这些信息将增加我们对石斑鱼幼虫变态发育过程的理解，并为幼虫发育期间抗菌策略的发展提供启示。
