Sweer Jordan A, Chen Mason T, Salimian Kevan J, Battafarano Richard J, Durr Nicholas J
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland.
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
J Biophotonics. 2019 Sep;12(9):e201900005. doi: 10.1002/jbio.201900005. Epub 2019 Jun 14.
As the incidence of esophageal adenocarcinoma continues to rise, there is a need for improved imaging technologies with contrast to abnormal esophageal tissues. To inform the design of optical technologies that meet this need, we characterize the spatial distribution of the scattering and absorption properties from 471 to 851 nm of eight resected human esophagi tissues using Spatial Frequency Domain Imaging. Histopathology was used to categorize tissue types, including normal, inflammation, fibrotic, ulceration, Barrett's Esophagus and squamous cell carcinoma. Average absorption and reduced scattering coefficients of normal tissues were 0.211 ± 0.051 and 1.20 ± 0.18 mm , respectively at 471 nm, and both values decreased monotonically with increasing wavelength. Fibrotic tissue exhibited at least 68% larger scattering signal across all wavelengths, while squamous cell carcinoma exhibited a 36% decrease in scattering at 471 nm. We additionally image the esophagus with high spatial frequencies up to 0.5 mm and show strong reflectance contrast to tissue treated with radiation. Lastly, we observe that esophageal absorption and scattering values change by an average of 9.4% and 2.7% respectively over a 30 minute duration post-resection. These results may guide system design for the diagnosis, prevention and monitoring of esophageal pathologies.
随着食管腺癌的发病率持续上升，需要有能与异常食管组织形成对比的改进型成像技术。为了为满足这一需求的光学技术设计提供参考，我们使用空间频域成像技术对8份切除的人体食管组织在471至851纳米范围内的散射和吸收特性的空间分布进行了表征。组织病理学用于对组织类型进行分类，包括正常、炎症、纤维化、溃疡、巴雷特食管和鳞状细胞癌。正常组织在471纳米处的平均吸收系数和约化散射系数分别为0.211±0.051和1.20±0.18毫米，并且这两个值均随波长增加而单调下降。纤维化组织在所有波长下的散射信号至少大68%，而鳞状细胞癌在471纳米处的散射减少36%。我们还对食管进行了高达0.5毫米的高空间频率成像，并显示出与接受放疗的组织有强烈的反射对比。最后，我们观察到在切除后的30分钟内，食管的吸收值和散射值分别平均变化9.4%和2.7%。这些结果可能会为食管疾病的诊断、预防和监测的系统设计提供指导。
